Human Pluripotent Stem Cell-Derived Intestinal Organoids Model SARS-CoV-2 Infection Revealing a Common Epithelial Inflammatory Response.
Stem Cell Reports
; 16(4): 940-953, 2021 04 13.
Article
in English
| MEDLINE | ID: covidwho-1180038
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leading to coronavirus disease 2019 (COVID-19) usually results in respiratory disease, but extrapulmonary manifestations are of major clinical interest. Intestinal symptoms of COVID-19 are present in a significant number of patients, and include nausea, diarrhea, and viral RNA shedding in feces. Human induced pluripotent stem cell-derived intestinal organoids (HIOs) represent an inexhaustible cellular resource that could serve as a valuable tool to study SARS-CoV-2 as well as other enteric viruses that infect the intestinal epithelium. Here, we report that SARS-CoV-2 productively infects both proximally and distally patterned HIOs, leading to the release of infectious viral particles while stimulating a robust transcriptomic response, including a significant upregulation of interferon-related genes that appeared to be conserved across multiple epithelial cell types. These findings illuminate a potential inflammatory epithelial-specific signature that may contribute to both the multisystemic nature of COVID-19 as well as its highly variable clinical presentation.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Organoids
/
Colon
/
COVID-19
/
Intestinal Mucosa
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Stem Cell Reports
Year:
2021
Document Type:
Article
Affiliation country:
J.stemcr.2021.02.019
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