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Comparison of the Quantitative DiaSorin Liaison Antigen Test to Reverse Transcription-PCR for the Diagnosis of COVID-19 in Symptomatic and Asymptomatic Outpatients.
Lefever, Stefanie; Indevuyst, Christophe; Cuypers, Lize; Dewaele, Klaas; Yin, Nicolas; Cotton, Frédéric; Padalko, Elizaveta; Oyaert, Matthijs; Descy, Julie; Cavalier, Etienne; Van Ranst, Marc; André, Emmanuel; Lagrou, Katrien; Vermeersch, Pieter.
  • Lefever S; Clinical Department of Laboratory Medicine and National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • Indevuyst C; Clinical Laboratory, Medisch Centrum Huisartsen, Leuven, Belgium.
  • Cuypers L; Clinical Department of Laboratory Medicine and National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • Dewaele K; Clinical Department of Laboratory Medicine and National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • Yin N; Department of Microbiology, LHUB-ULB, Université Libre de Bruxelles, Brussels, Belgium.
  • Cotton F; Department of Clinical Chemistry, LHUB-ULB, Université Libre de Bruxelles, Brussels, Belgium.
  • Padalko E; Department of Laboratory Medicine, Medical Microbiology, Ghent University Hospital, Ghent, Belgium.
  • Oyaert M; Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.
  • Descy J; Department of Clinical Microbiology and Center for Interdisciplinary Research on Medicines, University Hospital of Liège, Liège, Belgium.
  • Cavalier E; Department of Clinical Chemistry, University Hospital of Liège, Liège, Belgium.
  • Van Ranst M; Clinical Department of Laboratory Medicine and National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • André E; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.
  • Lagrou K; Clinical Department of Laboratory Medicine and National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Leuven, Belgium.
  • Vermeersch P; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.
J Clin Microbiol ; 59(7): e0037421, 2021 06 18.
Article in English | MEDLINE | ID: covidwho-1486479
ABSTRACT
We evaluated the quantitative DiaSorin Liaison severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen test in symptomatic and asymptomatic individuals consulting their general practitioners (GPs) during a period of stable intense virus circulation (213/100,000 habitants per day). Leftover reverse transcription-PCR (RT-PCR) positive (n = 204) and negative (n = 210) nasopharyngeal samples were randomly selected among fresh routine samples collected from patients consulting their GPs. Samples were tested on Liaison XL according to the manufacturer's instructions. Equivocal results were considered negative. The overall sensitivity and specificity of the Liaison antigen test compared to RT-PCR were 65.7% (95% confidence interval [CI], 58.9% to 71.9%) and 100% (CI, 97.8% to 100%). Sensitivity in samples with viral loads of ≥105, ≥104, and ≥103 copies/ml were 100% (CI, 96.3% to 100.0%), 96.5% (CI, 91.8% to 98.7%), and 87.4% (CI, 81.3% to 91.5%), respectively. All samples with ≤103 copies/ml were antigen negative. The ratio of antigen concentration to viral load in samples with ≥103 copies/ml was comparable in symptomatic and asymptomatic individuals (P = 0.58). The proportion of RT-PCR-positive participants with a high viral load (≥105 copies/ml) was not significantly higher in symptomatic than in asymptomatic participants (63.9% [CI, 54.9% to 72.0%] versus 51.9% [CI, 41.1% to 62.6%]; P = 0.11), but the proportion of participants with a low viral load (<103 copies/ml) was significantly higher in asymptomatic than in symptomatic RT-PCR-positive participants (35.4% [CI, 25.8% to 46.4%] versus 14.3% [CI, 9.0% to 21.8%]; P < 0.01). Sensitivity and specificity in samples with a viral load of ≥104 copies/ml were 96.5% and 100%. The correlation of antigen concentration with viral load was comparable in symptomatic and asymptomatic individuals.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: J Clin Microbiol Year: 2021 Document Type: Article Affiliation country: Jcm.00374-21

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: J Clin Microbiol Year: 2021 Document Type: Article Affiliation country: Jcm.00374-21