Essential requirement for JPT2 in NAADP-evoked Ca2+ signaling.
Sci Signal
; 14(675)2021 03 23.
Article
in English
| MEDLINE | ID: covidwho-1186203
ABSTRACT
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a "clickable" NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca2+ signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca2+ signaling and control of coronaviral entry.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Calcium Signaling
/
SARS-CoV-2
/
COVID-19
/
Microtubule-Associated Proteins
/
NADP
Limits:
Animals
/
Humans
Language:
English
Journal subject:
Science
/
Physiology
Year:
2021
Document Type:
Article
Affiliation country:
Scisignal.abd5605
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