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Essential requirement for JPT2 in NAADP-evoked Ca2+ signaling.
Gunaratne, Gihan S; Brailoiu, Eugen; He, Shijun; Unterwald, Ellen M; Patel, Sandip; Slama, James T; Walseth, Timothy F; Marchant, Jonathan S.
  • Gunaratne GS; Department of Pharmacology, University of Minnesota Medical School, 312 Church Street, Minneapolis, MN 55455, USA.
  • Brailoiu E; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
  • He S; Center for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA.
  • Unterwald EM; Department of Medicinal and Biological Chemistry, University of Toledo College of Pharmacy and Pharmaceutical Sciences, 3000 Arlington Avenue, Toledo, OH 43614, USA.
  • Patel S; Center for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA.
  • Slama JT; Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
  • Walseth TF; Center for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA.
  • Marchant JS; Department of Pharmacology, University of Minnesota Medical School, 312 Church Street, Minneapolis, MN 55455, USA.
Sci Signal ; 14(675)2021 03 23.
Article in English | MEDLINE | ID: covidwho-1186203
Semantic information from SemMedBD (by NLM)
1. JPT2 gene|JPT2 COEXISTS_WITH calcium ion
Subject
JPT2 gene|JPT2
Predicate
COEXISTS_WITH
Object
calcium ion
2. Organelles LOCATION_OF calcium ion
Subject
Organelles
Predicate
LOCATION_OF
Object
calcium ion
3. calcium ion AFFECTS endolysosome
Subject
calcium ion
Predicate
AFFECTS
Object
endolysosome
4. JPT2 gene|JPT2 PART_OF receptor complex
Subject
JPT2 gene|JPT2
Predicate
PART_OF
Object
receptor complex
5. JPT2 gene|JPT2 COEXISTS_WITH calcium ion
Subject
JPT2 gene|JPT2
Predicate
COEXISTS_WITH
Object
calcium ion
6. Organelles LOCATION_OF calcium ion
Subject
Organelles
Predicate
LOCATION_OF
Object
calcium ion
7. calcium ion AFFECTS endolysosome
Subject
calcium ion
Predicate
AFFECTS
Object
endolysosome
8. JPT2 gene|JPT2 PART_OF receptor complex
Subject
JPT2 gene|JPT2
Predicate
PART_OF
Object
receptor complex
ABSTRACT
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a "clickable" NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca2+ signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca2+ signaling and control of coronaviral entry.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Calcium Signaling / SARS-CoV-2 / COVID-19 / Microtubule-Associated Proteins / NADP Limits: Animals / Humans Language: English Journal subject: Science / Physiology Year: 2021 Document Type: Article Affiliation country: Scisignal.abd5605

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Calcium Signaling / SARS-CoV-2 / COVID-19 / Microtubule-Associated Proteins / NADP Limits: Animals / Humans Language: English Journal subject: Science / Physiology Year: 2021 Document Type: Article Affiliation country: Scisignal.abd5605