The Importance of Apparent pKa in the Development of Nanoparticles Encapsulating siRNA and mRNA.
Trends Pharmacol Sci
; 42(6): 448-460, 2021 06.
Article
in English
| MEDLINE | ID: covidwho-1187875
ABSTRACT
Polymer and lipid nanoparticles have been extensively used as carriers to address the biological barriers encountered in siRNA and mRNA delivery. We summarize the crucial role of nanoparticle charge and ionizability in complexing RNAs, binding to biological components, escaping from the endosome, and releasing RNAs into the cytoplasm. We highlight the significant impact of the apparent pKa of nanoparticles on their efficacy and toxicity, and the importance of optimizing pKa in the development of lead formulations for RNAs. We also discuss the feasibility of fine-tuning the pKa in nanoparticles and the applications of this approach in the optimization of delivery systems for RNAs.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Nanoparticles
Limits:
Humans
Language:
English
Journal:
Trends Pharmacol Sci
Year:
2021
Document Type:
Article
Affiliation country:
J.tips.2021.03.002
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