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Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection.
Inaba, Masato; Higashimoto, Yuki; Toyama, Yoko; Horiguchi, Tomoya; Hibino, Masaya; Iwata, Mitsunaga; Imaizumi, Kazuyoshi; Doi, Yohei.
  • Inaba M; Department of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. Electronic address: imasato@fujita-hu.ac.jp.
  • Higashimoto Y; Faculty of Medical Technology, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
  • Toyama Y; Department of Respiratory Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Horiguchi T; Department of Respiratory Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Hibino M; Department of Emergency and General Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Iwata M; Department of Emergency and General Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Imaizumi K; Department of Respiratory Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Doi Y; Department of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, Japan; Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Int J Infect Dis ; 107: 195-200, 2021 Jun.
Article in English | MEDLINE | ID: covidwho-1188630
ABSTRACT

OBJECTIVE:

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study aimed to determine the diagnostic sensitivity and specificity of RT-LAMP compared to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) over the disease course of COVID-19.

METHODS:

A total of 124 nasopharyngeal swab samples obtained from 24 COVID-19 patients were tested by RT-LAMP and RT-qPCR. Sensitivities and specificities of RT-LAMP compared with RT-qPCR were analyzed as a function of time from onset.

RESULTS:

Up to the 9th day after onset, the RT-LAMP had a positivity of 92.8%, and the sensitivity and specificity compared with RT-qPCR was 100%. However, after the 10th day after onset, the positivity of RT-LAMP decreased to less than 25%, and the concordance of positivity between the two methods was below 60%. The limit of detection of RT-LAMP was 6.7 copies/reaction.

CONCLUSIONS:

Until the 9th day after the onset of symptoms, RT-LAMP had the same diagnostic accuracy as RT-qPCR. These findings suggest that RT-LAMP can be used as a diagnostic tool for COVID-19 as an alternative to RT-qPCR in the acute symptomatic phase of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / COVID-19 Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / COVID-19 Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article