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N-terminally truncated nucleocapsid protein of SARS-CoV-2 as a better serological marker than whole nucleocapsid protein in evaluating the immunogenicity of inactivated SARS-CoV-2.
Yue, Lei; Cao, Han; Xie, Tianhong; Long, Runxiang; Li, Hua; Yang, Ting; Yan, Min; Xie, Zhongping.
  • Yue L; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Cao H; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Xie T; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Long R; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Li H; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Yang T; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
  • Yan M; Department of Microbiology and Immunology, Kunming Medical University, Kunming, Yunnan, China.
  • Xie Z; The Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, China.
J Med Virol ; 93(3): 1732-1738, 2021 03.
Article in English | MEDLINE | ID: covidwho-1196496
ABSTRACT
The coronavirus disease 2019 pandemic caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) had led to a serious public health crisis, and no specific treatments or vaccines are available yet. A nucleocapsid protein (NP)-based enzyme-linked immunosorbent assay (ELISA) detection method is not only important in disease diagnosis, but is required for the evaluation of vaccine efficacy during the development of an inactivated SARS-CoV-2 vaccine. In this study, we expressed both the NP and N-terminally truncated NP (ΔN-NP) of SARS-CoV-2 in an Escherichia coli expression system and described the purification of the soluble recombinant NP and ΔN-NP in details. The identities of the NP and ΔN-NP were confirmed with mass spectrometry. We then used immunoglobulin G detection ELISAs to compare the sensitivity of NP and ΔN-NP in detecting anti-SARS-CoV-2 antibodies. ΔN-NP showed greater sensitivity than NP in the analysis of serially diluted sera from mice and rabbits vaccinated with inactive SARS-CoV-2 and in human sera diluted 1400. ΔN-NP showed a positive detection rate similar to that of the SARS-CoV-2 S protein in human sera. We conclude that ΔN-NP is a better serological marker than NP for evaluating the immunogenicity of inactivated SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Inactivated / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines / SARS-CoV-2 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26541

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Inactivated / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines / SARS-CoV-2 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26541