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Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus.
Patel, Ami; Reuschel, Emma L; Xu, Ziyang; Zaidi, Faraz I; Kim, Kevin Y; Scott, Dana P; Mendoza, Janess; Ramos, Stephanie; Stoltz, Regina; Feldmann, Friederike; Okumura, Atsushi; Meade-White, Kimberly; Haddock, Elaine; Thomas, Tina; Rosenke, Rebecca; Lovaglio, Jamie; Hanley, Patrick W; Saturday, Greg; Muthumani, Kar; Feldmann, Heinz; Humeau, Laurent M; Broderick, Kate E; Weiner, David B.
  • Patel A; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Reuschel EL; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Xu Z; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Zaidi FI; Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
  • Kim KY; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Scott DP; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Mendoza J; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Ramos S; Inovio Pharmaceuticals Inc., Plymouth Meeting, Pennsylvania, USA.
  • Stoltz R; Inovio Pharmaceuticals Inc., Plymouth Meeting, Pennsylvania, USA.
  • Feldmann F; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Okumura A; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Meade-White K; Laboratory of Virology, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Haddock E; Laboratory of Virology, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Thomas T; Laboratory of Virology, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Rosenke R; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Lovaglio J; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Hanley PW; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Saturday G; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Muthumani K; Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Feldmann H; Vaccine & Immunotherapy Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
  • Humeau LM; Laboratory of Virology, Division of Intramural Research, National Institutes of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA.
  • Broderick KE; Inovio Pharmaceuticals Inc., Plymouth Meeting, Pennsylvania, USA.
  • Weiner DB; Inovio Pharmaceuticals Inc., Plymouth Meeting, Pennsylvania, USA.
JCI Insight ; 6(10)2021 05 24.
Article in English | MEDLINE | ID: covidwho-1197299
ABSTRACT
Emerging coronaviruses from zoonotic reservoirs, including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been associated with human-to-human transmission and significant morbidity and mortality. Here, we study both intradermal and intramuscular 2-dose delivery regimens of an advanced synthetic DNA vaccine candidate encoding a full-length MERS-CoV spike (S) protein, which induced potent binding and neutralizing antibodies as well as cellular immune responses in rhesus macaques. In a MERS-CoV challenge, all immunized rhesus macaques exhibited reduced clinical symptoms, lowered viral lung load, and decreased severity of pathological signs of disease compared with controls. Intradermal vaccination was dose sparing and more effective in this model at protecting animals from disease. The data support the further study of this vaccine for preventing MERS-CoV infection and transmission, including investigation of such vaccines and simplified delivery routes against emerging coronaviruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / Coronavirus Infections / Vaccines, DNA / Middle East Respiratory Syndrome Coronavirus / Macaca mulatta Type of study: Prognostic study Topics: Vaccines Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: Jci.insight.146082

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / Coronavirus Infections / Vaccines, DNA / Middle East Respiratory Syndrome Coronavirus / Macaca mulatta Type of study: Prognostic study Topics: Vaccines Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: Jci.insight.146082