Expression of SARS coronavirus 1 spike protein from a herpesviral vector induces innate immune signaling and neutralizing antibody responses.
Virology
; 559: 165-172, 2021 07.
Article
in English
| MEDLINE | ID: covidwho-1198236
ABSTRACT
SARS coronavirus 1 (SARS-CoV-1) causes a respiratory infection that can lead to acute respiratory distress characterized by inflammation and high levels of cytokines in the lung tissue. In this study we constructed a herpes simplex virus 1 replication-defective mutant vector expressing SARS-CoV-1 spike protein as a potential vaccine vector and to probe the effects of spike protein on host cells. The spike protein expressed from this vector is functional in that it localizes to the surface of infected cells and induces fusion of ACE2-expressing cells. In immunized mice, the recombinant vector induced antibodies that bind to spike protein in an ELISA assay and that show neutralizing activity. The spike protein expressed from this vector can induce the expression of cytokines in an ACE2-independent, MyD88-dependent process. These results argue that the SARS-CoV-1 spike protein intrinsically activates signaling pathways that induce cytokines and contribute directly to the inflammatory process of SARS.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Herpesvirus 1, Human
/
Severe acute respiratory syndrome-related coronavirus
/
Antibodies, Neutralizing
/
Spike Glycoprotein, Coronavirus
/
Immunity, Innate
Topics:
Vaccines
Limits:
Animals
/
Humans
Language:
English
Journal:
Virology
Year:
2021
Document Type:
Article
Affiliation country:
J.virol.2021.04.006
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