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Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection.
Chang, Jessie J-Y; Rawlinson, Daniel; Pitt, Miranda E; Taiaroa, George; Gleeson, Josie; Zhou, Chenxi; Mordant, Francesca L; De Paoli-Iseppi, Ricardo; Caly, Leon; Purcell, Damian F J; Stinear, Timothy P; Londrigan, Sarah L; Clark, Michael B; Williamson, Deborah A; Subbarao, Kanta; Coin, Lachlan J M.
  • Chang JJ; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Rawlinson D; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Pitt ME; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Taiaroa G; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbou
  • Gleeson J; Centre for Stem Cell Systems, Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Zhou C; Department of Clinical Pathology, University of Melbourne, Melbourne, VIC 3000, Australia.
  • Mordant FL; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • De Paoli-Iseppi R; Centre for Stem Cell Systems, Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Caly L; Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Purcell DFJ; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Stinear TP; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Londrigan SL; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia.
  • Clark MB; Centre for Stem Cell Systems, Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, VIC 3010, Australia.
  • Williamson DA; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; Department of Microbiology, Royal Melbourne Hospital, Melbourne, VIC 3050, Australia.
  • Subbarao K; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; WHO Collaborating Centre for Reference and Research on Influenza, Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Aus
  • Coin LJM; Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; Department of Clinical Pathology, University of Melbourne, Melbourne, VIC 3000, Australia; Department of Infectious Disease, Imperial College
Cell Rep ; 35(6): 109108, 2021 05 11.
Article in English | MEDLINE | ID: covidwho-1202346
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses subgenomic RNA (sgRNA) to produce viral proteins for replication and immune evasion. We apply long-read RNA and cDNA sequencing to in vitro human and primate infection models to study transcriptional dynamics. Transcription-regulating sequence (TRS)-dependent sgRNA upregulates earlier in infection than TRS-independent sgRNA. An abundant class of TRS-independent sgRNA consisting of a portion of open reading frame 1ab (ORF1ab) containing nsp1 joins to ORF10, and the 3' untranslated region (UTR) upregulates at 48 h post-infection in human cell lines. We identify double-junction sgRNA containing both TRS-dependent and -independent junctions. We find multiple sites at which the SARS-CoV-2 genome is consistently more modified than sgRNA and that sgRNA modifications are stable across transcript clusters, host cells, and time since infection. Our work highlights the dynamic nature of the SARS-CoV-2 transcriptome during its replication cycle.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Transcription, Genetic / SARS-CoV-2 / COVID-19 Limits: Animals / Humans Language: English Journal: Cell Rep Year: 2021 Document Type: Article Affiliation country: J.celrep.2021.109108

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Transcription, Genetic / SARS-CoV-2 / COVID-19 Limits: Animals / Humans Language: English Journal: Cell Rep Year: 2021 Document Type: Article Affiliation country: J.celrep.2021.109108