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Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection.
Franco-Martínez, Lorena; Tecles, Fernando; Torres-Cantero, Alberto; Bernal, Enrique; San Lázaro, Indra; Alcaraz, María José; Vicente-Romero, María R; Lamy, Elsa; Sánchez-Resalt, Cristina; Rubio, Camila P; Tvarijonaviciute, Asta; Martínez-Subiela, Silvia; Cerón, José J.
  • Franco-Martínez L; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
  • Tecles F; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
  • Torres-Cantero A; Preventive Medicine, Hospital Clínico Universitario Virgen de la Arrixaca, IMIB, Universidad de Murcia, Murcia, Spain.
  • Bernal E; Unit of Infectious Diseases, Hospital General Universitario Reina Sofía, Universidad De Murcia, Murcia, Spain.
  • San Lázaro I; Preventive Medicine, Hospital Clínico Universitario Virgen de la Arrixaca, IMIB, Universidad de Murcia, Murcia, Spain.
  • Alcaraz MJ; Unit of Infectious Diseases, Hospital General Universitario Reina Sofía, Universidad De Murcia, Murcia, Spain.
  • Vicente-Romero MR; Unit of Microbiology, Hospital General Universitario Reina Sofía, Universidad De Murcia, Murcia, Spain.
  • Lamy E; Mediterranean Institute for Agriculture, Environment and Development (MED), Advanced Research and Training Institute (IIFA), University of Évora, Évora, Portugal.
  • Sánchez-Resalt C; Sport Medicine Centre, University of Murcia, Espinardo, Murcia, Spain.
  • Rubio CP; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
  • Tvarijonaviciute A; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
  • Martínez-Subiela S; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
  • Cerón JJ; Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia, Spain.
Clin Chem Lab Med ; 59(9): 1592-1599, 2021 Aug 26.
Article in English | MEDLINE | ID: covidwho-1206211
ABSTRACT

OBJECTIVES:

The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection.

METHODS:

The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2.

RESULTS:

The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35).

CONCLUSIONS:

tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Saliva / Biological Assay / Biomarkers / Adenosine Deaminase / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2021 Document Type: Article Affiliation country: Cclm-2021-0324

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Saliva / Biological Assay / Biomarkers / Adenosine Deaminase / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2021 Document Type: Article Affiliation country: Cclm-2021-0324