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Reversed-engineered human alveolar lung-on-a-chip model.
Huang, Di; Liu, Tingting; Liao, Junlong; Maharjan, Sushila; Xie, Xin; Pérez, Montserrat; Anaya, Ingrid; Wang, Shiwei; Tirado Mayer, Alan; Kang, Zhixin; Kong, Weijia; Mainardi, Valerio Luca; Garciamendez-Mijares, Carlos Ezio; García Martínez, Germán; Moretti, Matteo; Zhang, Weijia; Gu, Zhongze; Ghaemmaghami, Amir M; Zhang, Yu Shrike.
  • Huang D; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Liu T; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, People's Republic of China.
  • Liao J; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Maharjan S; Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, People's Republic of China.
  • Xie X; Department of Laboratory Diagnosis, The 971th Hospital, Qingdao 266072, People's Republic of China.
  • Pérez M; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Anaya I; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
  • Wang S; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Tirado Mayer A; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Kang Z; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Kong W; Department of Biotechnological Engineering, Monterrey Institute of Technology and Higher Education, Monterrey, Nuevo León 64849, Mexico.
  • Mainardi VL; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Garciamendez-Mijares CE; Department of Biotechnological Engineering, Monterrey Institute of Technology and Higher Education, Monterrey, Nuevo León 64849, Mexico.
  • García Martínez G; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Moretti M; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Zhang W; Department of Mechatronics Engineering, Monterrey Institute of Technology and Higher Education, Monterrey, Nuevo León 64849, Mexico.
  • Gu Z; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Ghaemmaghami AM; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
  • Zhang YS; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139.
Proc Natl Acad Sci U S A ; 118(19)2021 05 11.
Article in English | MEDLINE | ID: covidwho-1214016
ABSTRACT
Here, we present a physiologically relevant model of the human pulmonary alveoli. This alveolar lung-on-a-chip platform is composed of a three-dimensional porous hydrogel made of gelatin methacryloyl with an inverse opal structure, bonded to a compartmentalized polydimethylsiloxane chip. The inverse opal hydrogel structure features well-defined, interconnected pores with high similarity to human alveolar sacs. By populating the sacs with primary human alveolar epithelial cells, functional epithelial monolayers are readily formed. Cyclic strain is integrated into the device to allow biomimetic breathing events of the alveolar lung, which, in addition, makes it possible to investigate pathological effects such as those incurred by cigarette smoking and severe acute respiratory syndrome coronavirus 2 pseudoviral infection. Our study demonstrates a unique method for reconstitution of the functional human pulmonary alveoli in vitro, which is anticipated to pave the way for investigating relevant physiological and pathological events in the human distal lung.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pulmonary Alveoli / Lab-On-A-Chip Devices / Models, Biological Limits: Humans Language: English Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pulmonary Alveoli / Lab-On-A-Chip Devices / Models, Biological Limits: Humans Language: English Year: 2021 Document Type: Article