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A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination.
Handtke, Stefan; Wolff, Martina; Zaninetti, Carlo; Wesche, Jan; Schönborn, Linda; Aurich, Konstanze; Ulm, Lena; Hübner, Nils-Olaf; Becker, Karsten; Thiele, Thomas; Greinacher, Andreas.
  • Handtke S; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Wolff M; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Zaninetti C; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Wesche J; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Schönborn L; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Aurich K; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Ulm L; Friedrich Loeffler-Institute of Medical Microbiology, and.
  • Hübner NO; Central Unit for Infection Prevention and Control, University Medicine Greifswald, Greifswald, Germany.
  • Becker K; Friedrich Loeffler-Institute of Medical Microbiology, and.
  • Thiele T; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
  • Greinacher A; Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine.
Blood ; 137(26): 3656-3659, 2021 07 01.
Article in English | MEDLINE | ID: covidwho-1215090
ABSTRACT
Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Autoantibodies / Immunoglobulin G / Platelet Factor 4 / Platelet Activation / Vaccination / Purpura, Thrombocytopenic, Idiopathic / Receptors, IgG / Flow Cytometry / COVID-19 Vaccines / SARS-CoV-2 Type of study: Diagnostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: Blood Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Autoantibodies / Immunoglobulin G / Platelet Factor 4 / Platelet Activation / Vaccination / Purpura, Thrombocytopenic, Idiopathic / Receptors, IgG / Flow Cytometry / COVID-19 Vaccines / SARS-CoV-2 Type of study: Diagnostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: Blood Year: 2021 Document Type: Article