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SARS-CoV-2 encoded microRNAs are involved in the process of virus infection and host immune response.
Liu, Zhi; Wang, Jianwei; Ge, Yiyue; Xu, Yuyu; Guo, Mengchen; Mi, Kai; Xu, Rui; Pei, Yang; Zhang, Qiankun; Luan, Xiaoting; Hu, Zhibin; Chi, Ying; Liu, Xingyin.
  • Liu Z; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Wang J; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Ge Y; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Xu Y; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Guo M; National Health Commission Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Center for Diseases Control and Prevention, Nanjing, Jiangsu 210009, China.
  • Mi K; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Xu R; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Pei Y; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Zhang Q; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Luan X; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Hu Z; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Chi Y; State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
  • Liu X; Department of Pathogen Biology-Microbiology Division, Key Laboratory of Pathogen of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
J Biomed Res ; 35(3): 216-227, 2021 Jan 29.
Article in English | MEDLINE | ID: covidwho-1219565
ABSTRACT
The outbreak of COVID-19 caused by SARS-CoV-2 is spreading worldwide, with the pathogenesis mostly unclear. Both virus and host-derived microRNA (miRNA) play essential roles in the pathology of virus infection. This study aims to uncover the mechanism for SARS-CoV-2 pathogenicity from the perspective of miRNA. We scanned the SARS-CoV-2 genome for putative miRNA genes and miRNA targets and conducted in vivo experiments to validate the virus-encoded miRNAs and their regulatory role on the putative targets. One of such virus-encoded miRNAs, MR147-3p, was overexpressed that resulted in significantly decreased transcript levels of all of the predicted targets in human, i.e., EXOC7, RAD9A, and TFE3 in the virus-infected cells. The analysis showed that the immune response and cytoskeleton organization are two of the most notable biological processes regulated by the infection-modulated miRNAs. Additionally, the genomic mutation of SARS-CoV-2 contributed to the changed miRNA repository and targets, suggesting a possible role of miRNAs in the attenuated phenotype of SARS-CoV-2 during its evolution. This study provided a comprehensive view of the miRNA-involved regulatory system during SARS-CoV-2 infection, indicating possible antiviral therapeutics against SARS-CoV-2 through intervening miRNA regulation.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: J Biomed Res Year: 2021 Document Type: Article Affiliation country: Jbr.35.20200154

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Prognostic study Language: English Journal: J Biomed Res Year: 2021 Document Type: Article Affiliation country: Jbr.35.20200154