Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes.
Nat Methods
; 18(5): 499-506, 2021 05.
Article
in English
| MEDLINE | ID: covidwho-1220210
ABSTRACT
Competitive coevolution between microbes and viruses has led to the diversification of CRISPR-Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of CRISPR-Cas ribonucleases from hypersaline samples, named Cas13X and Cas13Y. We engineered Cas13X.1 (775 aa) for RNA interference experiments in mammalian cell lines. We found Cas13X.1 could tolerate single-nucleotide mismatches in RNA recognition, facilitating prophylactic RNA virus inhibition. Moreover, a minimal RNA base editor, composed of engineered deaminase (385 aa) and truncated Cas13X.1 (445 aa), exhibited robust editing efficiency and high specificity to induce RNA base conversions. Our results suggest that there exist untapped bacterial defense systems in natural microbes that can function efficiently in mammalian cells, and thus potentially are useful for RNA-editing-based research.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA, Bacterial
/
RNA Editing
/
CRISPR-Cas Systems
Limits:
Animals
/
Humans
Language:
English
Journal:
Nat Methods
Journal subject:
Laboratory Techniques and procedures
Year:
2021
Document Type:
Article
Affiliation country:
S41592-021-01124-4
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