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Multichannel Immunosensor Platform for the Rapid Detection of SARS-CoV-2 and Influenza A(H1N1) Virus.
Li, Jianyong; Lin, Rui; Yang, Yi; Zhao, Rongtao; Song, Shiping; Zhou, Yi; Shi, Jiye; Wang, Lihua; Song, Hongbin; Hao, Rongzhang.
  • Li J; Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China.
  • Lin R; University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
  • Yang Y; Chinese PLA Center for Disease Control and Prevention, Beijing 100071, P. R. China.
  • Zhao R; Frontier Defence Medical Service Training Group, Army Medical University, Changji 831200, P. R. China.
  • Song S; Chinese PLA Center for Disease Control and Prevention, Beijing 100071, P. R. China.
  • Zhou Y; Chinese PLA Center for Disease Control and Prevention, Beijing 100071, P. R. China.
  • Shi J; The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, P. R. China.
  • Wang L; College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P. R. China.
  • Song H; Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China.
  • Hao R; University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
ACS Appl Mater Interfaces ; 13(19): 22262-22270, 2021 May 19.
Article in English | MEDLINE | ID: covidwho-1221187
ABSTRACT
The coronavirus disease 2019 (COVID-19) can present a similar syndrome to an influenza infection, which may complicate diagnosis and clinical management of these two important respiratory infectious diseases, especially during the peak season of influenza. A rapid and convenient point-of-care test (POCT) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza virus is of great importance for prompt and efficient control of these respiratory epidemics. Herein, a multichannel electrochemical immunoassay (MEIA) platform was developed based on a disposable screen-printed carbon electrode (SPCE) array for the on-site detection of SARS-CoV-2 and A(H1N1). The developed MEIA was constructed with eight channels and allowed rapid detection on a single array. On the SPCE surface, monoclonal antibodies against influenza A(H1N1) hemagglutinin (HA) protein or SARS-CoV-2 spike protein were coated to capture the target antigens, which then interacted with a horseradish peroxidase (HRP)-labeled detection antibody to form an immuno-sandwich complex. The results showed that the MEIA exhibited a broader linear range than ELISA and comparable sensitivity for A(H1N1) HA and SARS-CoV-2 spike protein. The detection results on 79 clinical samples for A(H1N1) suggested that the proposed MEIA platform showed comparable results with ELISA in sensitivity (with a positive rate of 100% for positive samples) but higher specificity, with a false-positive rate of 5.4% for negative samples versus that of 40.5% with ELISA. Thus, it offers great potential for the on-the-spot differential diagnosis of infected patients, which would significantly benefit the efficient control and prevent the spread of these infectious diseases in communities or resource-limited regions in the future.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Biosensing Techniques / Influenza, Human / Influenza A Virus, H1N1 Subtype / Electrochemical Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Biosensing Techniques / Influenza, Human / Influenza A Virus, H1N1 Subtype / Electrochemical Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2021 Document Type: Article