A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2.
RNA Biol
; 18(12): 2218-2225, 2021 12.
Article
in English
| MEDLINE | ID: covidwho-1221426
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
Early detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proven crucial during the efforts to mitigate the effects of the COVID-19 pandemic. Several diagnostic methods have emerged in the past few months, each with different shortcomings and limitations. The current gold standard, RT-qPCR using fluorescent probes, relies on demanding equipment requirements plus the high costs of the probes and specific reaction mixes. To broaden the possibilities of reagents and thermocyclers that could be allocated towards this task, we have optimized an alternative strategy for RT-qPCR diagnosis. This is based on a widely used DNA-intercalating dye and can be implemented with several different qPCR reagents and instruments. Remarkably, the proposed qPCR method performs similarly to the broadly used TaqMan-based detection, in terms of specificity and sensitivity, thus representing a reliable tool. We think that, through enabling the use of vast range of thermocycler models and laboratory facilities for SARS-CoV-2 diagnosis, the alternative proposed here can increase dramatically the testing capability, especially in countries with limited access to costly technology and reagents.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Quinolines
/
RNA, Viral
/
Diamines
/
Benzothiazoles
/
Real-Time Polymerase Chain Reaction
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
/
COVID-19
/
Intercalating Agents
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
RNA Biol
Journal subject:
Molecular Biology
Year:
2021
Document Type:
Article
Affiliation country:
15476286.2021.1926648
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