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Salivary Biomarkers in COVID-19 Patients: Towards a Wide-Scale Test for Monitoring Disease Activity.
Napodano, Cecilia; Callà, Cinzia; Fiorita, Antonella; Marino, Mariapaola; Taddei, Eleonora; Di Cesare, Tiziana; Passali, Giulio Cesare; Di Santo, Riccardo; Stefanile, Annunziata; Fantoni, Massimo; Urbani, Andrea; Paludetti, Gaetano; Rapaccini, Gian Ludovico; Ciasca, Gabriele; Basile, Umberto.
  • Napodano C; Dipartimento di Scienze Mediche e Chirurgiche, UOC Gastroenterologia, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Callà C; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Fiorita A; Dipartimento di Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa e del Collo, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Marino M; Dipartimento di Medicina e Chirurgia Traslazionale, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Taddei E; Dipartimento di Sicurezza e Bioetica, Sezione di Malattie Infettive, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Di Cesare T; Dipartimento di Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa e del Collo, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Passali GC; Dipartimento di Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa e del Collo, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Di Santo R; Dipartimento di Neuroscienze, Sezione di Fisica, Fondazione Policlinico A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Stefanile A; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Fantoni M; Dipartimento di Sicurezza e Bioetica, Sezione di Malattie Infettive, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Urbani A; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Paludetti G; Dipartimento di Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa e del Collo, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Rapaccini GL; Dipartimento di Scienze Mediche e Chirurgiche, UOC Gastroenterologia, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Ciasca G; Dipartimento di Neuroscienze, Sezione di Fisica, Fondazione Policlinico A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
  • Basile U; Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
J Pers Med ; 11(5)2021 May 08.
Article in English | MEDLINE | ID: covidwho-1224056
Semantic information from SemMedBD (by NLM)
1. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
2. 2019 novel coronavirus PROCESS_OF Patients
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
Patients
3. Cohort LOCATION_OF IgA1|IGHA1
Subject
Cohort
Predicate
LOCATION_OF
Object
IgA1|IGHA1
4. Cohort LOCATION_OF IgA2
Subject
Cohort
Predicate
LOCATION_OF
Object
IgA2
5. Cohort LOCATION_OF Immunoglobulin A subclass
Subject
Cohort
Predicate
LOCATION_OF
Object
Immunoglobulin A subclass
6. IgA1|IGHA1 compared_with IgA2
Subject
IgA1|IGHA1
Predicate
compared_with
Object
IgA2
7. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
8. 2019 novel coronavirus PROCESS_OF Patients
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
Patients
9. Cohort LOCATION_OF IgA1|IGHA1
Subject
Cohort
Predicate
LOCATION_OF
Object
IgA1|IGHA1
10. Cohort LOCATION_OF IgA2
Subject
Cohort
Predicate
LOCATION_OF
Object
IgA2
11. Cohort LOCATION_OF Immunoglobulin A subclass
Subject
Cohort
Predicate
LOCATION_OF
Object
Immunoglobulin A subclass
12. IgA1|IGHA1 compared_with IgA2
Subject
IgA1|IGHA1
Predicate
compared_with
Object
IgA2
ABSTRACT
The ongoing outbreak of coronavirus disease 2019 (COVID-19), which impairs the functionality of several organs, represents a major threat to human health. One of the hardest challenges in the fight against COVID-19 is the development of wide-scale, effective, and rapid laboratory tests to control disease severity, progression, and possible sudden worsening. Monitoring patients in real-time is highly demanded in this pandemic era when physicians need reliable and quantitative tools to prioritize patients' access to intensive care departments. In this regard, salivary biomarkers are extremely promising, as they allow for the fast and non-invasive collection of specimens and can be repeated multiple times.

METHODS:

We compare salivary levels of immunoglobulin A subclasses (IgA1 and IgA2) and free light chains (kFLC and λFLC) in a cohort of 29 SARS-CoV-2 patients and 21 healthy subjects.

RESULTS:

We found that each biomarker differs significantly between the two groups, with p-values ranging from 10-8 to 10-4. A Receiving Operator Curve analysis shows that λFLC level is the best-suited candidate to discriminate the two groups (AUC = 0.96), with an accuracy of 0.94 (0.87-1.00 95% CI), a precision of 0.91 (0.81-1.00 95% CI), a sensitivity of 1.00 (0.96-1.00 95% CI), and a specificity of 0.86 (0.70-1.00 95% CI).

CONCLUSION:

These results suggest λFLC as an ideal indicator of patient conditions. This hypothesis is strengthened by the consideration that the λFLC half-life (approximately 6 h) is significantly shorter than the IgA one (21 days), thus confirming the potential of λFLC for effectively monitoring patients' fluctuation in real-time.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Language: English Year: 2021 Document Type: Article Affiliation country: Jpm11050385

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Language: English Year: 2021 Document Type: Article Affiliation country: Jpm11050385