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SARS-Cov-2 spike protein fragment 674-685 protects mitochondria from releasing cytochrome c in response to apoptogenic influence.
Kalashnyk, Olena; Lykhmus, Olena; Izmailov, Mykhailo; Koval, Lyudmyla; Komisarenko, Serhiy; Skok, Maryna.
  • Kalashnyk O; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine.
  • Lykhmus O; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine.
  • Izmailov M; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine.
  • Koval L; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine.
  • Komisarenko S; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine.
  • Skok M; Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine. Electronic address: skok@biochem.kiev.ua.
Biochem Biophys Res Commun ; 561: 14-18, 2021 07 05.
Article in English | MEDLINE | ID: covidwho-1225147
ABSTRACT
In spite of numerous studies, many details of SARS-Cov-2 interaction with human cells are still poorly understood. The 674-685 fragment of SARS-Cov-2 spike protein is homologous to the fragment of α-cobratoxin underlying its interaction with α7 nicotinic acetylcholine receptors (nAChRs). The interaction of 674-685 peptide with α7 nAChR has been predicted in silico. In the present paper we confirm this prediction experimentally and investigate the effect of SARS-Cov-2 spike protein peptide on mitochondria, which express α7 nAChRs to regulate apoptosis-related events. We demonstrate that SARS-Cov-2 spike protein peptide 674-685 competes with the antibody against 179-190 fragment of α7 nAChR subunit for the binding to α7-expressing cells and mitochondria and prevents the release of cytochrome c from isolated mitochondria in response to 0.5 mM H2O2 but does not protect intact U373 cells against apoptogenic effect of H2O2. Our data suggest that the α7 nAChR-binding portion of SARS-Cov-2 spike protein prevents mitochondria-driven apoptosis when the virus is uncoated inside the cell and, therefore, supports the infected cell viability before the virus replication cycle is complete.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptide Fragments / Apoptosis / Cytochromes c / Spike Glycoprotein, Coronavirus / Mitochondria Type of study: Prognostic study Limits: Animals Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2021.05.018

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptide Fragments / Apoptosis / Cytochromes c / Spike Glycoprotein, Coronavirus / Mitochondria Type of study: Prognostic study Limits: Animals Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2021.05.018