Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2.
J Virol Methods
; 294: 114183, 2021 08.
Article
in English
| MEDLINE | ID: covidwho-1225322
ABSTRACT
BACKGROUND:
The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the worldwide coronavirus disease-19 (COVID-19) pandemic, starting in late 2019. The standard diagnostic methods to detect SARS-CoV-2 are PCR-based genetic assays. Antigen-antibody-based immunochromatographic assays are alternative methods of detecting this virus. Rapid diagnosis kits to detect SARS-CoV-2 are urgently needed. STUDYDESIGN:
Three monoclonal antibodies against SARS-CoV-2 nucleocapsid (N) protein were used to develop an antigen-antibody-based immunochromatographic kit to detect SARS-CoV-2. These assays were evaluated usingã nasopharyngeal swab specimens collected from patients suspected of having COVID-19.RESULTS:
These assays detected recombinant SARS-CoV-2â¯N protein at concentrations >0.2â¯ng/mL within 10â¯min after protein loading, but did not detect the N proteins of Middle East respiratory syndrome coronavirus (MERS-CoV), human coronaviruses OC43 (HCoV-OC43) and 299E (HCoV-229E) and other pathogens causing respiratory infections. Nasopharyngeal swab specimens obtained 1ï½3, 4ï½9, and ≥ 10 days after symptom onset from COVID-19 patients diagnosed by RT-PCR showed positivity rates of 100 %, >80 %, and <30 %, respectively.CONCLUSIONS:
Kits using this immunochromatographic assay may be a rapid and useful tool for point-of-care diagnosis of COVID-19 when samples are obtained from patients 1ï½9 days after symptom onset.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Immunoassay
/
Coronavirus Nucleocapsid Proteins
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
Topics:
Long Covid
Limits:
Animals
/
Humans
Language:
English
Journal:
J Virol Methods
Year:
2021
Document Type:
Article
Affiliation country:
J.jviromet.2021.114183
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