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Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy.
Alkhalifa, Haifa; Mohammed, Fatima; Taurin, Sebastien; Greish, Khaled; Taha, Safa; Fredericks, Salim.
  • Alkhalifa H; Department of Basic Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, Adliya 15503, Kingdom of Bahrain.
  • Mohammed F; Department of Science, New York University Abu Dhabi, Abu Dhabi 129188, United Arab Emirates.
  • Taurin S; Department of Basic Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, Adliya 15503, Kingdom of Bahrain.
  • Greish K; Department of Molecular Medicine, College of Medicine and Medical Sciences, Princess Al-Jawhara Centre for Molecular Medicine, Arabian Gulf University, Segaya, Manama 328, Kingdom of Bahrain.
  • Taha S; Department of Molecular Medicine, College of Medicine and Medical Sciences, Princess Al-Jawhara Centre for Molecular Medicine, Arabian Gulf University, Segaya, Manama 328, Kingdom of Bahrain.
  • Fredericks S; Department of Molecular Medicine, College of Medicine and Medical Sciences, Princess Al-Jawhara Centre for Molecular Medicine, Arabian Gulf University, Segaya, Manama 328, Kingdom of Bahrain.
Oncol Lett ; 21(6): 458, 2021 Jun.
Article in English | MEDLINE | ID: covidwho-1225869
Semantic information from SemMedBD (by NLM)
1. Cryosurgery TREATS Malignant Neoplasms
Subject
Cryosurgery
Predicate
TREATS
Object
Malignant Neoplasms
2. water channel AFFECTS export across plasma membrane
Subject
water channel
Predicate
AFFECTS
Object
export across plasma membrane
3. Assay USES lissamine rhodamine B
Subject
Assay
Predicate
USES
Object
lissamine rhodamine B
4. Cell Count MEASURES Assay
Subject
Cell Count
Predicate
MEASURES
Object
Assay
5. Freezing AFFECTS cell injury
Subject
Freezing
Predicate
AFFECTS
Object
cell injury
6. AQP3 gene|AQP3 compared_with RN
Subject
AQP3 gene|AQP3
Predicate
compared_with
Object
RN
7. MCF-7 Cells LOCATION_OF AQP3 gene|AQP3
Subject
MCF-7 Cells
Predicate
LOCATION_OF
Object
AQP3 gene|AQP3
8. MCF-7 Cells LOCATION_OF AQP5 gene|AQP5
Subject
MCF-7 Cells
Predicate
LOCATION_OF
Object
AQP5 gene|AQP5
9. Cryosurgery TREATS Malignant Neoplasms
Subject
Cryosurgery
Predicate
TREATS
Object
Malignant Neoplasms
10. water channel AFFECTS export across plasma membrane
Subject
water channel
Predicate
AFFECTS
Object
export across plasma membrane
11. Assay USES lissamine rhodamine B
Subject
Assay
Predicate
USES
Object
lissamine rhodamine B
12. Cell Count MEASURES Assay
Subject
Cell Count
Predicate
MEASURES
Object
Assay
13. Freezing AFFECTS cell injury
Subject
Freezing
Predicate
AFFECTS
Object
cell injury
14. AQP3 gene|AQP3 compared_with RNA, Small Interfering
Subject
AQP3 gene|AQP3
Predicate
compared_with
Object
RNA, Small Interfering
15. MCF-7 Cells LOCATION_OF AQP3 gene|AQP3
Subject
MCF-7 Cells
Predicate
LOCATION_OF
Object
AQP3 gene|AQP3
16. MCF-7 Cells LOCATION_OF AQP5 gene|AQP5
Subject
MCF-7 Cells
Predicate
LOCATION_OF
Object
AQP5 gene|AQP5
ABSTRACT
Cryoablation is an emerging type of treatment for cancer. The sensitization of tumors using cryosensitizing agents prior to treatment enhances ablation efficiency and may improve clinical outcomes. Water efflux, which is regulated by aquaporin channels, contributes to cancer cell damage achieved through cryoablation. An increase in aquaporin (AQP) 3 is cryoprotective, whereas its inhibition augments cryodamage. The present study aimed to investigate aquaporin (AQP1, AQP3 and AQP5) gene expression and cellular localization in response to cryoinjury. Cultured breast cancer cells (MDA-MB-231 and MCF-7) were exposed to freezing to induce cryoinjury. RNA and protein extracts were then analyzed using reverse transcription-quantitative PCR and western blotting, respectively. Localization of aquaporins was studied using immunocytochemistry. Additionally, cells were transfected with small interfering RNA to silence aquaporin gene expression and cell viability was assessed using the Sulforhodamine B assay. Cryoinjury did not influence gene expression of AQPs, except for a 4-fold increase of AQP1 expression in MDA-MD-231 cells. There were no clear differences in AQP protein expression for either cell lines upon exposure to frozen and non-frozen temperatures, with the exception of fainter AQP5 bands for non-frozen MCF-7 cells. The exposure of cancer cells to freezing temperatures altered the localization of AQP1 and AQP3 proteins in both MCF-7 and MDA-MD-231 cells. The silencing of AQP1, AQP3 and AQP5 exacerbated MDA-MD-231 cell damage associated with freezing compared with control siRNA. This was also observed with AQP3 and AQP5 silencing in MCF-7 cells. Inhibition of aquaporins may potentially enhance cryoinjury. This cryosensitizing process may be used as an adjunct to breast cancer cryotherapy, especially in the border area targeted by cryoablation where freezing temperatures are not cold enough to induce cellular damage.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Oncol Lett Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Oncol Lett Year: 2021 Document Type: Article