Your browser doesn't support javascript.
Impact of lipid nanoparticle size on mRNA vaccine immunogenicity.
Hassett, Kimberly J; Higgins, Jaclyn; Woods, Angela; Levy, Becca; Xia, Yan; Hsiao, Chiaowen Joyce; Acosta, Edward; Almarsson, Örn; Moore, Melissa J; Brito, Luis A.
  • Hassett KJ; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Higgins J; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Woods A; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Levy B; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Xia Y; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Hsiao CJ; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Acosta E; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Almarsson Ö; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Moore MJ; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America.
  • Brito LA; Moderna, Inc, 200 Technology Square, Cambridge, MA 02139, United States of America. Electronic address: Luis.Brito@Modernatx.com.
J Control Release ; 335: 237-246, 2021 07 10.
Article in English | MEDLINE | ID: covidwho-1233005
ABSTRACT
Lipid nanoparticles (LNP) are effective delivery vehicles for messenger RNA (mRNA) and have shown promise for vaccine applications. Yet there are no published reports detailing how LNP biophysical properties can impact vaccine performance. In our hands, a retrospective analysis of mRNA LNP vaccine in vivo studies revealed a relationship between LNP particle size and immunogenicity in mice using LNPs of various compositions. To further investigate this, we designed a series of studies to systematically change LNP particle size without altering lipid composition and evaluated biophysical properties and immunogenicity of the resulting LNPs. While small diameter LNPs were substantially less immunogenic in mice, all particle sizes tested yielded a robust immune response in non-human primates (NHP).
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanoparticles / Immunogenicity, Vaccine Type of study: Experimental Studies / Observational study Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Control Release Journal subject: Pharmacology Year: 2021 Document Type: Article Affiliation country: J.jconrel.2021.05.021

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanoparticles / Immunogenicity, Vaccine Type of study: Experimental Studies / Observational study Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Control Release Journal subject: Pharmacology Year: 2021 Document Type: Article Affiliation country: J.jconrel.2021.05.021