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DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist.
Thépaut, Michel; Luczkowiak, Joanna; Vivès, Corinne; Labiod, Nuria; Bally, Isabelle; Lasala, Fátima; Grimoire, Yasmina; Fenel, Daphna; Sattin, Sara; Thielens, Nicole; Schoehn, Guy; Bernardi, Anna; Delgado, Rafael; Fieschi, Franck.
  • Thépaut M; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Luczkowiak J; Instituto de Investigación Hospital Universitario 12 de Octubre (imas12), Universidad Complutense School of Medicine, Madrid, Spain.
  • Vivès C; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Labiod N; Instituto de Investigación Hospital Universitario 12 de Octubre (imas12), Universidad Complutense School of Medicine, Madrid, Spain.
  • Bally I; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Lasala F; Instituto de Investigación Hospital Universitario 12 de Octubre (imas12), Universidad Complutense School of Medicine, Madrid, Spain.
  • Grimoire Y; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Fenel D; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Sattin S; Universita`degli Studi di Milano, Dipartimento di Chimica, Milano, Italy.
  • Thielens N; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Schoehn G; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
  • Bernardi A; Universita`degli Studi di Milano, Dipartimento di Chimica, Milano, Italy.
  • Delgado R; Instituto de Investigación Hospital Universitario 12 de Octubre (imas12), Universidad Complutense School of Medicine, Madrid, Spain.
  • Fieschi F; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.
PLoS Pathog ; 17(5): e1009576, 2021 05.
Article in English | MEDLINE | ID: covidwho-1236599
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
The efficient spread of SARS-CoV-2 resulted in a unique pandemic in modern history. Despite early identification of ACE2 as the receptor for viral spike protein, much remains to be understood about the molecular events behind viral dissemination. We evaluated the contribution of C-type lectin receptors (CLRS) of antigen-presenting cells, widely present in respiratory mucosa and lung tissue. DC-SIGN, L-SIGN, Langerin and MGL bind to diverse glycans of the spike using multiple interaction areas. Using pseudovirus and cells derived from monocytes or T-lymphocytes, we demonstrate that while virus capture by the CLRs examined does not allow direct cell infection, DC/L-SIGN, among these receptors, promote virus transfer to permissive ACE2+ Vero E6 cells. A glycomimetic compound designed against DC-SIGN, enable inhibition of this process. These data have been then confirmed using authentic SARS-CoV-2 virus and human respiratory cell lines. Thus, we described a mechanism potentiating viral spreading of infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Lectins, C-Type / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 Type of study: Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: PLoS Pathog Year: 2021 Document Type: Article Affiliation country: Journal.ppat.1009576

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Lectins, C-Type / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 Type of study: Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: PLoS Pathog Year: 2021 Document Type: Article Affiliation country: Journal.ppat.1009576