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CRISPR is a useful biological tool for detecting nucleic acid of SARS-CoV-2 in human clinical samples.
Rahman, Md Rashidur; Hossain, Md Amjad; Mozibullah, Md; Mujib, Fateh Al; Afrose, Afrina; Shahed-Al-Mahmud, Md; Apu, Md Aminul Islam.
  • Rahman MR; Department of Chemical Engineering, Kyushu University, Japan; Department of Pharmacy, Jashore University of Science and Technology, Bangladesh. Electronic address: mr.rahman@just.edu.bd.
  • Hossain MA; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Bangladesh.
  • Mozibullah M; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Bangladesh.
  • Mujib FA; Department of Pharmacy, Mawlana Bhashani Science and Technology University, Bangladesh.
  • Afrose A; Department of Pharmacy, BRAC University, Bangladesh.
  • Shahed-Al-Mahmud M; Department of Microbiology and Immunology, School of Medicine, Tzu Chi University, Hualien, Taiwan; Genomics Research Center, Academia Sinica, Taipei, Taiwan.
  • Apu MAI; Graduate School of International Agricultural Technology, Seoul National University, South Korea.
Biomed Pharmacother ; 140: 111772, 2021 Aug.
Article in English | MEDLINE | ID: covidwho-1244709
ABSTRACT
The recent pandemic of novel coronavirus disease (COVID-19) has spread globally and infected millions of people. The quick and specific detection of the nucleic acid of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) remains a challenge within healthcare providers. Currently, quantitative reverse transcription-polymerase chain reaction (RT-qPCR) is the widely used method to detect the SARS-CoV-2 from the human clinical samples. RT-qPCR is expensive equipment and needs skilled personnel as well as lengthy detection time. RT-qPCR limitation needed an alternative healthcare technique to overcome with a fast and cheaper detection method. By applying the principles of CRISPR technology, several promising detection methods giving hope to the healthcare community. CRISPR-based detection methods include SHERLOCK-Covid, STOP-Covid, AIOD-CRISPR, and DETECTR platform. These methods have comparative advantages and drawbacks. Among these methods, AIOD-CRISPR and DETECTR are reasonably better diagnostic methods than the others if we compare the time taken for the test, the cost associated with each test, and their capability of detecting SARS-CoV-2 in the clinical samples. It may expect that the promising CRISPR-based methods would facilitate point-of-care (POC) applications in the CRISPR-built next-generation novel coronavirus diagnostics.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR-Cas Systems / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biomed Pharmacother Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR-Cas Systems / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biomed Pharmacother Year: 2021 Document Type: Article