Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2.
Chem Commun (Camb)
; 57(51): 6229-6232, 2021 Jun 24.
Article
in English
| MEDLINE | ID: covidwho-1246405
ABSTRACT
Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Oligonucleotides, Antisense
/
Spectroscopy, Near-Infrared
/
Quantum Dots
/
Fluorescent Dyes
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chem Commun (Camb)
Journal subject:
Chemistry
Year:
2021
Document Type:
Article
Affiliation country:
D1cc01410b
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