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Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2.
Moitra, Parikshit; Alafeef, Maha; Dighe, Ketan; Sheffield, Zach; Dahal, Dipendra; Pan, Dipanjan.
  • Moitra P; Center for Blood Oxygen Transport and Hemostasis, Department of Pediatrics, University of Maryland Baltimore School of Medicine, 670 W Baltimore St., Baltimore, Maryland 21201, USA. dipanjan@som.umaryland.edu.
  • Alafeef M; Center for Blood Oxygen Transport and Hemostasis, Department of Pediatrics, University of Maryland Baltimore School of Medicine, 670 W Baltimore St., Baltimore, Maryland 21201, USA. dipanjan@som.umaryland.edu and Department of Chemical, Biochemical and Environmental Engineering, University of Maryla
  • Dighe K; Center for Blood Oxygen Transport and Hemostasis, Department of Pediatrics, University of Maryland Baltimore School of Medicine, 670 W Baltimore St., Baltimore, Maryland 21201, USA. dipanjan@som.umaryland.edu and Department of Chemical, Biochemical and Environmental Engineering, University of Maryla
  • Sheffield Z; Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, 1000 Hiltop Circle, Baltimore, Maryland 21250, USA.
  • Dahal D; Center for Blood Oxygen Transport and Hemostasis, Department of Pediatrics, University of Maryland Baltimore School of Medicine, 670 W Baltimore St., Baltimore, Maryland 21201, USA. dipanjan@som.umaryland.edu.
  • Pan D; Center for Blood Oxygen Transport and Hemostasis, Department of Pediatrics, University of Maryland Baltimore School of Medicine, 670 W Baltimore St., Baltimore, Maryland 21201, USA. dipanjan@som.umaryland.edu and Department of Chemical, Biochemical and Environmental Engineering, University of Maryla
Chem Commun (Camb) ; 57(51): 6229-6232, 2021 Jun 24.
Article in English | MEDLINE | ID: covidwho-1246405
ABSTRACT
Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Oligonucleotides, Antisense / Spectroscopy, Near-Infrared / Quantum Dots / Fluorescent Dyes / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: D1cc01410b

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Oligonucleotides, Antisense / Spectroscopy, Near-Infrared / Quantum Dots / Fluorescent Dyes / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: D1cc01410b