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Epidemiological Correlates of Polymerase Chain Reaction Cycle Threshold Values in the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Salvatore, Phillip P; Dawson, Patrick; Wadhwa, Ashutosh; Rabold, Elizabeth M; Buono, Sean; Dietrich, Elizabeth A; Reses, Hannah E; Vuong, Jeni; Pawloski, Lucia; Dasu, Trivikram; Bhattacharyya, Sanjib; Pevzner, Eric; Hall, Aron J; Tate, Jacqueline E; Kirking, Hannah L.
  • Salvatore PP; Coronavirus Disease 2019 (COVID-19) Response Team, Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Dawson P; Coronavirus Disease 2019 (COVID-19) Response Team, Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Wadhwa A; Coronavirus Disease 2019 (COVID-19) Response Team, Laboratory Leadership Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Rabold EM; Coronavirus Disease 2019 (COVID-19) Response Team, Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Buono S; Coronavirus Disease 2019 (COVID-19) Response Team, Laboratory Leadership Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Dietrich EA; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Reses HE; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Vuong J; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Pawloski L; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Dasu T; City of Milwaukee Health Department, Milwaukee, Wisconsin, USA.
  • Bhattacharyya S; City of Milwaukee Health Department, Milwaukee, Wisconsin, USA.
  • Pevzner E; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Hall AJ; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Tate JE; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Kirking HL; Coronavirus Disease 2019 (COVID-19) Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Clin Infect Dis ; 72(11): e761-e767, 2021 06 01.
Article in English | MEDLINE | ID: covidwho-1249288
Semantic information from SemMedBD (by NLM)
1. upper respiratory symptom PROCESS_OF Participant
Subject
upper respiratory symptom
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2. Symptoms PROCESS_OF Persons
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Symptoms
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3. 2019 novel coronavirus PROCESS_OF Persons
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2019 novel coronavirus
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4. upper respiratory symptom PROCESS_OF Participant
Subject
upper respiratory symptom
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PROCESS_OF
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5. Symptoms PROCESS_OF Persons
Subject
Symptoms
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Persons
6. 2019 novel coronavirus PROCESS_OF Persons
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2019 novel coronavirus
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ABSTRACT

BACKGROUND:

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has principally been performed through the use of real-time reverse-transcription polymerase chain reaction testing. Results of such tests can be reported as cycle threshold (Ct) values, which may provide semi-quantitative or indirect measurements of viral load. Previous reports have examined temporal trends in Ct values over the course of a SARS-CoV-2 infection.

METHODS:

Using testing data collected during a prospective household transmission investigation of outpatient and mild coronavirus disease 2019 cases, we examined the relationships between Ct values of the viral RNA N1 target and demographic, clinical, and epidemiological characteristics collected through participant interviews and daily symptom diaries.

RESULTS:

We found that Ct values are lowest (corresponding to a higher viral RNA concentration) soon after symptom onset and are significantly correlated with the time elapsed since onset (P < .001); within 7 days after symptom onset, the median Ct value was 26.5, compared with a median Ct value of 35.0 occurring 21 days after onset. Ct values were significantly lower among participants under 18 years of age (P = .01) and those reporting upper respiratory symptoms at the time of sample collection (P = .001), and were higher among participants reporting no symptoms (P = .05).

CONCLUSIONS:

These results emphasize the importance of early testing for SARS-CoV-2 among individuals with symptoms of respiratory illness, and allow cases to be identified and isolated when their viral shedding may be highest.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Adolescent / Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: Cid

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Adolescent / Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: Cid