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A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens.
Wang, Xinye; Xiu, Leshan; Binder, Raquel A; Toh, Teck-Hock; Lee, Jeffrey Soon-Yit; Ting, Jakie; Than, Son T; Qi, Wenhao; Coleman, Kristen K; Perera, David; Ma, Maijuan; Gray, Gregory C.
  • Wang X; Global Health Research Center, Duke Kunshan University, Kunshan, China.
  • Xiu L; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
  • Binder RA; Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA.
  • Toh TH; Duke Global Health Institute, Duke University, Durham, NC, USA.
  • Lee JS; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
  • Ting J; Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA.
  • Than ST; Duke Global Health Institute, Duke University, Durham, NC, USA.
  • Qi W; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia.
  • Coleman KK; Clinical Research Center, Sibu Hospital, Ministry of Health Malaysia, Sibu, Sarawak, Malaysia.
  • Perera D; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia.
  • Ma M; Clinical Research Center, Sibu Hospital, Ministry of Health Malaysia, Sibu, Sarawak, Malaysia.
  • Gray GC; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia.
One Health ; 13: 100274, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1251441
ABSTRACT
We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: One Health Year: 2021 Document Type: Article Affiliation country: J.onehlt.2021.100274

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: One Health Year: 2021 Document Type: Article Affiliation country: J.onehlt.2021.100274