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Measurement of Cellular Immune Response to Viral Infection and Vaccination.
Bouwman, Wilbert; Verhaegh, Wim; Holtzer, Laurent; van de Stolpe, Anja.
  • Bouwman W; Philips Research, Eindhoven, Netherlands.
  • Verhaegh W; Philips Research, Eindhoven, Netherlands.
  • Holtzer L; Philips Molecular Pathway Dx, Eindhoven, Netherlands.
  • van de Stolpe A; Philips Molecular Pathway Dx, Eindhoven, Netherlands.
Front Immunol ; 11: 575074, 2020.
Article in English | MEDLINE | ID: covidwho-1256374
Semantic information from SemMedBD (by NLM)
1. Blood specimen ASSOCIATED_WITH Infected
Subject
Blood specimen
Predicate
ASSOCIATED_WITH
Object
Infected
2. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
3. Blood specimen PART_OF Patients
Subject
Blood specimen
Predicate
PART_OF
Object
Patients
4. Bacterial Infections COEXISTS_WITH Influenza
Subject
Bacterial Infections
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COEXISTS_WITH
Object
Influenza
5. Janus kinase|STAT3 ASSOCIATED_WITH Severe infection
Subject
Janus kinase|STAT3
Predicate
ASSOCIATED_WITH
Object
Severe infection
6. Vaccine PART_OF C0029341
Subject
Vaccine
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PART_OF
Object
C0029341
7. Blood specimen LOCATION_OF yellow fever vaccine
Subject
Blood specimen
Predicate
LOCATION_OF
Object
yellow fever vaccine
8. Blood specimen ASSOCIATED_WITH Infected
Subject
Blood specimen
Predicate
ASSOCIATED_WITH
Object
Infected
9. Infected PROCESS_OF Patients
Subject
Infected
Predicate
PROCESS_OF
Object
Patients
10. Blood specimen PART_OF Patients
Subject
Blood specimen
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PART_OF
Object
Patients
11. Bacterial Infections COEXISTS_WITH Influenza
Subject
Bacterial Infections
Predicate
COEXISTS_WITH
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Influenza
12. Janus kinase|STAT3 ASSOCIATED_WITH Severe infection
Subject
Janus kinase|STAT3
Predicate
ASSOCIATED_WITH
Object
Severe infection
13. Vaccines, Inactivated PART_OF Orthomyxoviridae
Subject
Vaccines, Inactivated
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PART_OF
Object
Orthomyxoviridae
14. Blood specimen LOCATION_OF yellow fever vaccine
Subject
Blood specimen
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LOCATION_OF
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yellow fever vaccine
ABSTRACT
Combined cellular and humoral host immune response determine the clinical course of a viral infection and effectiveness of vaccination, but currently the cellular immune response cannot be measured on simple blood samples. As functional activity of immune cells is determined by coordinated activity of signaling pathways, we developed mRNA-based JAK-STAT signaling pathway activity assays to quantitatively measure the cellular immune response on Affymetrix expression microarray data of various types of blood samples from virally infected patients (influenza, RSV, dengue, yellow fever, rotavirus) or vaccinated individuals, and to determine vaccine immunogenicity. JAK-STAT1/2 pathway activity was increased in blood samples of patients with viral, but not bacterial, infection and was higher in influenza compared to RSV-infected patients, reflecting known differences in immunogenicity. High JAK-STAT3 pathway activity was associated with more severe RSV infection. In contrast to inactivated influenza virus vaccine, live yellow fever vaccine did induce JAK-STAT1/2 pathway activity in blood samples, indicating superior immunogenicity. Normal (healthy) JAK-STAT1/2 pathway activity was established, enabling assay interpretation without the need for a reference sample. The JAK-STAT pathway assays enable measurement of cellular immune response for prognosis, therapy stratification, vaccine development, and clinical testing.
Subject(s)
Dengue Virus/immunology; Immunity, Cellular; Orthomyxoviridae/immunology; Respiratory Syncytial Virus, Human/immunology; Rotavirus/immunology; Viral Vaccines/therapeutic use; Virus Diseases/immunology; Yellow fever virus/immunology; Biomarkers/blood; Dengue/blood; Dengue/immunology; Dengue/prevention & control; Dengue/virology; Dengue Vaccines/therapeutic use; Dengue Virus/pathogenicity; Diagnosis, Differential; Host-Pathogen Interactions; Humans; Immunogenicity, Vaccine; Influenza Vaccines/therapeutic use; Influenza, Human/blood; Influenza, Human/immunology; Influenza, Human/prevention & control; Influenza, Human/virology; Oligonucleotide Array Sequence Analysis; Orthomyxoviridae/pathogenicity; Predictive Value of Tests; RNA, Messenger/blood; RNA, Messenger/genetics; Respiratory Syncytial Virus Infections/blood; Respiratory Syncytial Virus Infections/immunology; Respiratory Syncytial Virus Infections/prevention & control; Respiratory Syncytial Virus Infections/virology; Respiratory Syncytial Virus, Human/pathogenicity; Rotavirus/pathogenicity; Rotavirus Infections/blood; Rotavirus Infections/immunology; Rotavirus Infections/prevention & control; Rotavirus Infections/virology; Rotavirus Vaccines; Signal Transduction/genetics; Virus Diseases/blood; Virus Diseases/prevention & control; Virus Diseases/virology; Yellow Fever/blood; Yellow Fever/immunology; Yellow Fever/prevention & control; Yellow Fever/virology; Yellow Fever Vaccine/therapeutic use; Yellow fever virus/pathogenicity
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Orthomyxoviridae / Yellow fever virus / Virus Diseases / Viral Vaccines / Respiratory Syncytial Virus, Human / Rotavirus / Dengue Virus / Immunity, Cellular Subject: Orthomyxoviridae / Yellow fever virus / Virus Diseases / Viral Vaccines / Respiratory Syncytial Virus, Human / Rotavirus / Dengue Virus / Immunity, Cellular Language: English Journal: Front Immunol Clinical aspect: Diagnosis / Prediction / Prognosis / Therapy Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Orthomyxoviridae / Yellow fever virus / Virus Diseases / Viral Vaccines / Respiratory Syncytial Virus, Human / Rotavirus / Dengue Virus / Immunity, Cellular Subject: Orthomyxoviridae / Yellow fever virus / Virus Diseases / Viral Vaccines / Respiratory Syncytial Virus, Human / Rotavirus / Dengue Virus / Immunity, Cellular Language: English Journal: Front Immunol Clinical aspect: Diagnosis / Prediction / Prognosis / Therapy Year: 2020
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