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Applications of Deep Mutational Scanning in Virology.
Burton, Thomas D; Eyre, Nicholas S.
  • Burton TD; College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia.
  • Eyre NS; College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia.
Viruses ; 13(6)2021 05 28.
Article in English | MEDLINE | ID: covidwho-1256665
ABSTRACT
Several recently developed high-throughput techniques have changed the field of molecular virology. For example, proteomics studies reveal complete interactomes of a viral protein, genome-wide CRISPR knockout and activation screens probe the importance of every single human gene in aiding or fighting a virus, and ChIP-seq experiments reveal genome-wide epigenetic changes in response to infection. Deep mutational scanning is a relatively novel form of protein science which allows the in-depth functional analysis of every nucleotide within a viral gene or genome, revealing regions of importance, flexibility, and mutational potential. In this review, we discuss the application of this technique to RNA viruses including members of the Flaviviridae family, Influenza A Virus and Severe Acute Respiratory Syndrome Coronavirus 2. We also briefly discuss the reverse genetics systems which allow for analysis of viral replication cycles, next-generation sequencing technologies and the bioinformatics tools that facilitate this research.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA Viruses / Sequence Analysis, RNA / High-Throughput Nucleotide Sequencing / Mutation Language: English Year: 2021 Document Type: Article Affiliation country: V13061020

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA Viruses / Sequence Analysis, RNA / High-Throughput Nucleotide Sequencing / Mutation Language: English Year: 2021 Document Type: Article Affiliation country: V13061020