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Human Coronavirus NL63 Among Other Respiratory Viruses in Clinical Specimens of Egyptian Children and Raw Sewage Samples.
El-Senousy, Waled Morsy; Shouman, Mohamed.
  • El-Senousy WM; Environmental Virology Lab, Water Pollution Research Department, Environmental Research Division and Food-Borne Viruses Group, Centre of Excellence for Advanced Sciences, National Research Centre (NRC), 33 El-Buhouth st., Dokki, Giza, 12622, Egypt. waledmorsy@hotmail.com.
  • Shouman M; Pediatric Department, Centre of Medical Excellence, Medical Research Division, NRC, Dokki, Giza, Egypt.
Food Environ Virol ; 13(3): 322-328, 2021 09.
Article in English | MEDLINE | ID: covidwho-1257868
ABSTRACT
The objective of this study was to investigate human coronavirus NL63 (HCoV-NL63) prevalence among the other respiratory viruses such as parainfluenza, respiratory syncytial virus, and non-enteric adenoviruses in clinical specimens of Egyptian children and raw sewage samples. One hundred clinical specimens were collected from Egyptian children suffering from upper and lower respiratory viral infections in the years 2005-2006 to detect HCoV-NL63 genome using RT-PCR. All the specimens were negative for the virus. Also, a complete absence of HCoV-NL63 genome was observed in the twenty-four raw sewage samples collected from two wastewater treatment plants within Greater Cairo from February 2006 to January 2007. Using nested RT-PCR, parainfluenza virus type 1, respiratory syncytial virus type A, adenovirus type 4, and adenovirus type 7 were detected in 3%, 2%, 5%, and 2% of the clinical specimens, respectively. Of these viruses, only adenovirus type 4 was detected in 1/24 (4.17%) of the raw sewage samples, while a complete absence of the other investigated respiratory viruses was observed in the raw sewage samples. The low percentage of positivity in the clinical specimens, the concentration method of the raw sewage samples, and the indirect routes of transmission may be the reasons for the absence of respiratory viruses in raw sewage samples. On the other hand, enteric adenoviruses were detected in 21/24 (87.5%) of the raw sewage samples with a higher prevalence of adenovirus type 41 than adenovirus type 40. A direct route of transmission of enteric viruses to raw sewage may be the reason for the high positivity percentage of enteric adenoviruses in raw sewage samples.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Tract Infections / Sewage / Adenoviridae / Respiratory Syncytial Virus, Human / Coronavirus Infections / Parainfluenza Virus 1, Human / Coronavirus NL63, Human Type of study: Observational study / Prognostic study Limits: Child, preschool / Humans / Infant Country/Region as subject: Africa Language: English Journal: Food Environ Virol Year: 2021 Document Type: Article Affiliation country: S12560-021-09479-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Tract Infections / Sewage / Adenoviridae / Respiratory Syncytial Virus, Human / Coronavirus Infections / Parainfluenza Virus 1, Human / Coronavirus NL63, Human Type of study: Observational study / Prognostic study Limits: Child, preschool / Humans / Infant Country/Region as subject: Africa Language: English Journal: Food Environ Virol Year: 2021 Document Type: Article Affiliation country: S12560-021-09479-9