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A Cross-Sectional Study of SARS-CoV-2 Seroprevalence between Fall 2020 and February 2021 in Allegheny County, Western Pennsylvania, USA.
Xu, Lingqing; Doyle, Joshua; Barbeau, Dominique J; Le Sage, Valerie; Wells, Alan; Duprex, W Paul; Shurin, Michael R; Wheeler, Sarah E; McElroy, Anita K.
  • Xu L; Division of Infectious Diseases, Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Doyle J; Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Barbeau DJ; Division of Infectious Diseases, Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Le Sage V; Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Wells A; Division of Infectious Diseases, Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Duprex WP; Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Shurin MR; Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Wheeler SE; Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • McElroy AK; Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15261, USA.
Pathogens ; 10(6)2021 Jun 06.
Article in English | MEDLINE | ID: covidwho-1259563
ABSTRACT
Seroprevalence studies are important for understanding the dynamics of local virus transmission and evaluating community immunity. To assess the seroprevalence for SARS-CoV-2 in Allegheny County, an urban/suburban county in Western PA, 393 human blood samples collected in Fall 2020 and February 2021 were examined for spike protein receptor-binding domain (RBD) and nucleocapsid protein (N) antibodies. All RBD-positive samples were evaluated for virus-specific neutralization activity. Our results showed a seroprevalence of 5.5% by RBD ELISA, 4.5% by N ELISA, and 2.5% for both in Fall 2020, which increased to 24.7% by RBD ELISA, 14.9% by N ELISA, and 12.9% for both in February 2021. Neutralization titer was significantly correlated with RBD titer but not with N titer. Using these two assays, we were able to distinguish infected from vaccinated individuals. In the February cohort, higher median income and white race were associated with serological findings consistent with vaccination. This study demonstrates a 4.5-fold increase in SARS-CoV-2 seroprevalence from Fall 2020 to February 2021 in Allegheny County, PA, due to increased incidence of both natural disease and vaccination. Future seroprevalence studies will need to include the effect of vaccination on assay results and incorporate non-vaccine antigens in serological assessments.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Pathogens10060710

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Pathogens10060710