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Complementary methods for SARS-CoV-2 diagnosis in times of material shortage.
Sandri, Thaisa Lucas; Inoue, Juliana; Geiger, Johanna; Griesbaum, Johanna-Marie; Heinzel, Constanze; Burnet, Michael; Fendel, Rolf; Kremsner, Peter G; Held, Jana; Kreidenweiss, Andrea.
  • Sandri TL; Institute of Tropical Medicine, Eberhard Karls University Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
  • Inoue J; Synovo GmbH, Paul-Ehrlich-Strasse 15, 72076, Tübingen, Germany.
  • Geiger J; Laboratory of Molecular Immunopathology, Department of Clinical Pathology, Federal University of Paraná, Curitiba, Brazil.
  • Griesbaum JM; Institute of Tropical Medicine, Eberhard Karls University Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
  • Heinzel C; Synovo GmbH, Paul-Ehrlich-Strasse 15, 72076, Tübingen, Germany.
  • Burnet M; Institute of Tropical Medicine, Eberhard Karls University Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
  • Fendel R; Institute of Tropical Medicine, Eberhard Karls University Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
  • Kremsner PG; Synovo GmbH, Paul-Ehrlich-Strasse 15, 72076, Tübingen, Germany.
  • Held J; Institute of Tropical Medicine, Eberhard Karls University Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
  • Kreidenweiss A; German Center for Infection Research (DZIF), Partner Site Tübingen, Wilhelmstrasse 27, 72074, Tübingen, Germany.
Sci Rep ; 11(1): 11899, 2021 06 07.
Article in English | MEDLINE | ID: covidwho-1260951
ABSTRACT
The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2-particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / RNA, Viral / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-91457-z

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / RNA, Viral / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-91457-z