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Mutations in SARS-CoV-2 nsp7 and nsp8 proteins and their predicted impact on replication/transcription complex structure.
Reshamwala, Shamlan M S; Likhite, Vishakha; Degani, Mariam S; Deb, Shalini S; Noronha, Santosh B.
  • Reshamwala SMS; DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Likhite V; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Degani MS; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Deb SS; DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Noronha SB; Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India.
J Med Virol ; 93(7): 4616-4619, 2021 07.
Article in English | MEDLINE | ID: covidwho-1263086
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) has been identified to be a mutation hot spot, with the P323L mutation being commonly observed in viral genomes isolated from North America. RdRp forms a complex with nonstructural proteins nsp7 and nsp8 to form the minimal replication/transcription machinery required for genome replication. As mutations in RdRp may affect formation of the RdRp-nsp7-nsp8 supercomplex, we analyzed viral genomes to identify mutations in nsp7 and nsp8 protein sequences. Based on in silico analysis of predicted structures of the supercomplex comprising of native and mutated proteins, we demonstrate that specific mutations in nsp7 and nsp8 proteins may have a role in stabilization of the replication/transcription complex.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Nonstructural Proteins / Coronavirus RNA-Dependent RNA Polymerase / Viral Replication Compartments / SARS-CoV-2 Type of study: Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26791

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Nonstructural Proteins / Coronavirus RNA-Dependent RNA Polymerase / Viral Replication Compartments / SARS-CoV-2 Type of study: Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26791