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Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost.
Matsuda, Elaine Monteiro; de Campos, Ivana Barros; de Oliveira, Isabela Penteriche; Colpas, Daniela Rodrigues; Carmo, Andreia Moreira Dos Santos; Brígido, Luís Fernando de Macedo.
  • Matsuda EM; Infectious Diseases Outpatient Clinic, Santo André Health Secretary, Santo André, São Paulo, Brazil.
  • de Campos IB; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
  • de Oliveira IP; Bioprocess and Biotecnology Engeneering, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Colpas DR; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
  • Carmo AMDS; Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil.
  • Brígido LFM; Virology Center, Adolfo Lutz Institute, São Paulo, São Paulo, Brazil.
J Med Virol ; 93(7): 4405-4410, 2021 07.
Article in English | MEDLINE | ID: covidwho-1263105
ABSTRACT
One year into the coronavirus disease 2019 (COVID-19) pandemic, diagnostic strategies, although central for contact tracing and other preventive measures, are still limited. To meet the global demand, lower cost and faster antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection are a convenient alternative to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay. We tested laboratory-based RT-PCR RNA detection and two rapid antigen detection (RAD) tests, based on the immunochromatography test for nucleocapsid protein of SARS-CoV-2 (COVID-19 Ag ECO Test, ECO Diagnóstica, and Panbio COVID-19 Ag Rapid Test Abbott). Paired collection and testing were done in a small prospective open study in three clinical services in São Paulo, constituted of mostly symptomatic volunteers at collection (97%, 109/112) for a median of 4 days (interquartile range 3-6), ranging from 1 to 30. Among the 108 paired RT-PCR/RAD tests, results were concordant in 96.4% (101/108). The test's performance was comparable, with an overall sensitivity of 87% and a specificity of 96%. These observations add to other data that suggest that antigen tests may provide reasonable sensitivity and specificity and deserve a role to improve testing strategies, especially in resource-limited settings.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Nucleic Acid Testing / COVID-19 Serological Testing / COVID-19 / Antigens, Viral Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Country/Region as subject: South America / Brazil Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26985

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Nucleic Acid Testing / COVID-19 Serological Testing / COVID-19 / Antigens, Viral Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Country/Region as subject: South America / Brazil Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.26985