Conformational Dynamics in the Interaction of SARS-CoV-2 Papain-like Protease with Human Interferon-Stimulated Gene 15 Protein.
J Phys Chem Lett
; 12(23): 5608-5615, 2021 Jun 17.
Article
in English
| MEDLINE | ID: covidwho-1263456
ABSTRACT
Papain-like protease (PLpro) from SARS-CoV-2 plays essential roles in the replication cycle of the virus. In particular, it preferentially interacts with and cleaves human interferon-stimulated gene 15 (hISG15) to suppress the innate immune response of the host. We used small-angle X-ray and neutron scattering combined with computational techniques to study the mechanism of interaction of SARS-CoV-2 PLpro with hISG15. We showed that hISG15 undergoes a transition from an extended to a compact state after binding to PLpro, a conformation that has not been previously observed in complexes of SARS-CoV-2 PLpro with ISG15 from other species. Furthermore, computational analysis showed significant conformational flexibility in the ISG15 N-terminal domain, suggesting that it is weakly bound to PLpro and supports a binding mechanism that is dominated by the C-terminal ISG15 domain. This study fundamentally improves our understanding of the SARS-CoV-2 deISGylation complex that will help guide development of COVID-19 therapeutics targeting this complex.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Ubiquitins
/
Cytokines
/
Interferons
/
Coronavirus Papain-Like Proteases
/
SARS-CoV-2
Limits:
Humans
Language:
English
Journal:
J Phys Chem Lett
Year:
2021
Document Type:
Article
Affiliation country:
Acs.jpclett.1c00831
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