Single-cell epigenomic landscape of peripheral immune cells reveals establishment of trained immunity in individuals convalescing from COVID-19.
Nat Cell Biol
; 23(6): 620-630, 2021 06.
Article
in English
| MEDLINE | ID: covidwho-1263492
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection often causes severe complications and even death. However, asymptomatic infection has also been reported, highlighting the difference in immune responses among individuals. Here we performed single-cell chromatin accessibility and T cell-receptor analyses of peripheral blood mononuclear cells collected from individuals convalescing from COVID-19 and healthy donors. Chromatin remodelling was observed in both innate and adaptive immune cells in the individuals convalescing from COVID-19. Compared with healthy donors, recovered individuals contained abundant TBET-enriched CD16+ and IRF1-enriched CD14+ monocytes with sequential trained and activated epigenomic states. The B-cell lineage in recovered individuals exhibited an accelerated developmental programme from immature B cells to antibody-producing plasma cells. Finally, an integrated analysis of single-cell T cell-receptor clonality with the chromatin accessibility landscape revealed the expansion of putative SARS-CoV-2-specific CD8+ T cells with epigenomic profiles that promote the differentiation of effector or memory cells. Overall, our data suggest that immune cells of individuals convalescing from COVID-19 exhibit global remodelling of the chromatin accessibility landscape, indicative of the establishment of immunological memory.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Monocytes
/
Lymphocyte Subsets
/
Genes, T-Cell Receptor
/
Epigenesis, Genetic
/
Single-Cell Analysis
/
Epigenomics
/
SARS-CoV-2
/
COVID-19
/
Immunologic Memory
Type of study:
Observational study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
/
Young adult
Language:
English
Journal:
Nat Cell Biol
Year:
2021
Document Type:
Article
Affiliation country:
S41556-021-00690-1
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