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Molecular mechanism of anti-SARS-CoV2 activity of Ashwagandha-derived withanolides.
Dhanjal, Jaspreet Kaur; Kumar, Vipul; Garg, Sukant; Subramani, Chandru; Agarwal, Shubhra; Wang, Jia; Zhang, Huayue; Kaul, Ashish; Kalra, Rajkumar Singh; Kaul, Sunil C; Vrati, Sudhanshu; Sundar, Durai; Wadhwa, Renu.
  • Dhanjal JK; Indraprastha Institute of Information Technology Delhi, Okhla Industrial Estate, Phase III, New Delhi 110 020, India. Electronic address: jaspreet@iiitd.ac.in.
  • Kumar V; DAILAB, Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110 016, India. Electronic address: vipul.kumar@dbeb.iitd.ac.in.
  • Garg S; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: sukant.garg@aist.go.jp.
  • Subramani C; Regional Centre for Biotechnology (RCB), Faridabad 121 001, India. Electronic address: s.chandru@rcb.res.in.
  • Agarwal S; Regional Centre for Biotechnology (RCB), Faridabad 121 001, India. Electronic address: shubhra.agarwal@rcb.res.in.
  • Wang J; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: wang-jia0819@aist.go.jp.
  • Zhang H; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: zhang-huayue@aist.go.jp.
  • Kaul A; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: ashish-kaul@aist.go.jp.
  • Kalra RS; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: rajkumar-singh@oist.jp.
  • Kaul SC; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: s-kaul@aist.go.jp.
  • Vrati S; Regional Centre for Biotechnology (RCB), Faridabad 121 001, India. Electronic address: vrati@rcb.res.in.
  • Sundar D; DAILAB, Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110 016, India. Electronic address: sundar@dbeb.iitd.ac.in.
  • Wadhwa R; AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305 8565, Japan. Electronic address: renu-wadhwa@aist.go.jp.
Int J Biol Macromol ; 184: 297-312, 2021 Aug 01.
Article in English | MEDLINE | ID: covidwho-1265684
Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
2. proline PART_OF KLK6 protei
Subject
proline
Predicate
PART_OF
Object
KLK6 protei
3. Withanolides INHIBITS proline
Subject
Withanolides
Predicate
INHIBITS
Object
proline
4. Withanolides INHIBITS TMPRSS2 gene|TMPRSS2
Subject
Withanolides
Predicate
INHIBITS
Object
TMPRSS2 gene|TMPRSS2
5. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
6. proline PART_OF KLK6 protein, human|KLK6
Subject
proline
Predicate
PART_OF
Object
KLK6 protein, human|KLK6
7. Withanolides INHIBITS proline
Subject
Withanolides
Predicate
INHIBITS
Object
proline
8. Withanolides INHIBITS TMPRSS2 gene|TMPRSS2
Subject
Withanolides
Predicate
INHIBITS
Object
TMPRSS2 gene|TMPRSS2
ABSTRACT
COVID-19 caused by SARS-CoV-2 corona virus has become a global pandemic. In the absence of drugs and vaccine, and premises of time, efforts and cost required for their development, natural resources such as herbs are anticipated to provide some help and may also offer a promising resource for drug development. Here, we have investigated the therapeutic prospective of Ashwagandha for the COVID-19 pandemic. Nine withanolides were tested in silico for their potential to target and inhibit (i) cell surface receptor protein (TMPRSS2) that is required for entry of virus to host cells and (ii) viral protein (the main protease Mpro) that is essential for virus replication. We report that the withanolides possess capacity to inhibit the activity of TMPRSS2 and Mpro. Furthermore, withanolide-treated cells showed downregulation of TMPRSS2 expression and inhibition of SARS-CoV-2 replication in vitro, suggesting that Ashwagandha may provide a useful resource for COVID-19 treatment.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Serine Endopeptidases / Plant Extracts / Viral Matrix Proteins / Withanolides / SARS-CoV-2 Topics: Traditional medicine / Vaccines Limits: Humans Language: English Journal: Int J Biol Macromol Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Serine Endopeptidases / Plant Extracts / Viral Matrix Proteins / Withanolides / SARS-CoV-2 Topics: Traditional medicine / Vaccines Limits: Humans Language: English Journal: Int J Biol Macromol Year: 2021 Document Type: Article