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Comparison of SARS-CoV-2 serological assays for use in epidemiological surveillance in Scotland.
McDonald, Lindsay; Wise, Helen; Muecksch, Frauke; Poston, Daniel; Mavin, Sally; Templeton, Kate; Furrie, Elizabeth; Richardson, Claire; McGuire, Jaqueline; Jarvis, Lisa; Malloy, Kristen; McAuley, Andrew; Palmateer, Norah; Dickson, Elizabeth; Hatziioannou, Theodora; Bieniasz, Paul; Jenks, Sara.
  • McDonald L; Royal Infirmary of Edinburgh, NHS Lothian, Edinburgh, Scotland.
  • Wise H; Royal Infirmary of Edinburgh, NHS Lothian, Edinburgh, Scotland.
  • Muecksch F; Laboratory of Retrovirology, The Rockefeller University, New York, New York, USA.
  • Poston D; Laboratory of Retrovirology, The Rockefeller University, New York, New York, USA.
  • Mavin S; Scottish Microbiology Reference Laboratory, NHS Highland, Inverness, Scotland.
  • Templeton K; Royal Infirmary of Edinburgh, NHS Lothian, Edinburgh, Scotland.
  • Furrie E; Ninewells Hospital and Medical School, NHS Tayside, Dundee, Scotland.
  • Richardson C; University Hospital Monklands, NHS Lanarkshire, Airdrie, Scotland.
  • McGuire J; University Hospital Monklands, NHS Lanarkshire, Airdrie, Scotland.
  • Jarvis L; Scottish National Blood Transfusion Service, The Jack Copland Centre, Edinburgh, Scotland.
  • Malloy K; Scottish National Blood Transfusion Service, The Jack Copland Centre, Edinburgh, Scotland.
  • McAuley A; Public Health Scotland, Glasgow, Scotland.
  • Palmateer N; School of Health & Life Sciences, Glasgow Caledonian University, Glasgow, Scotland.
  • Dickson E; Public Health Scotland, Glasgow, Scotland.
  • Hatziioannou T; School of Health & Life Sciences, Glasgow Caledonian University, Glasgow, Scotland.
  • Bieniasz P; Public Health Scotland, Glasgow, Scotland.
  • Jenks S; Laboratory of Retrovirology, The Rockefeller University, New York, New York, USA.
J Clin Virol Plus ; 1(3): 100028, 2021 Sep.
Article in English | MEDLINE | ID: covidwho-1267742
Preprint
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ABSTRACT

Background:

Sero-surveillance of SARS-CoV-2 is crucial to monitoring levels of population exposure and informing public health responses, but may be influenced by variability in performance between available assays.

Methods:

Five commercial immunoassays and a neutralising activity assay were used to detect antibodies to SARS-CoV-2 in routine primary care and paediatric samples collected during the first wave of the pandemic in NHS Lothian, Scotland as part of ongoing surveillance efforts. For each assay, sensitivity and specificity was calculated relative to consensus results (majority of immunoassays positive = overall positive) and neutralising activity. Quantitative correlation was performed between serological and neutralising titres.

Results:

Seroprevalence ranged from 3.4-7.3 % in primary care patients and 3-5.9 % in paediatric patients according to different immunoassays. Neutralising activity was detectable in 2.8 % and 1.3 % respectively. Relative assay performance changed depending on comparison to immunoassay consensus versus neutralising activity and qualititative versus quantitative agreement. Cross-reactivity with endemic seasonal coronaviruses was confirmed by neutralising assay in false positives for one immunoassay. Presence of false positives for another assay was found specifically in paediatric but not adult samples.

Conclusions:

Five serological assays show variable accuracy when applied to the general population, impacting seroprevalence estimates. Assay performance may also vary in detection of protective neutralising antibody levels. These aspects should be considered in assay selection and interpretation in epidemiological studies.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Observational study / Randomized controlled trials Language: English Journal: J Clin Virol Plus Year: 2021 Document Type: Article Affiliation country: J.jcvp.2021.100028

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Observational study / Randomized controlled trials Language: English Journal: J Clin Virol Plus Year: 2021 Document Type: Article Affiliation country: J.jcvp.2021.100028