Chloroquine attenuates thymic stromal lymphopoietin production via suppressing caspase-1 signaling in mast cells.
Biomed Pharmacother
; 141: 111835, 2021 Sep.
Article
in English
| MEDLINE | ID: covidwho-1271574
ABSTRACT
Thymic stromal lymphopoietin (TSLP) produced by mast cells is involved in allergic inflammation pathogenesis. Chloroquine (CQ) is known to be an anti-malarial drug; however, additional protective functions of CQ have been discovered. This study aims to clarify an anti-inflammatory effect of CQ through modulating TSLP levels using an in vitro model of phorbol myristate acetate (PMA) + A23187-activated human mast cell line (HMC-1) and an in vivo model of PMA-irritated ear edema. CQ treatment reduced the production and mRNA expression levels of TSLP in activated HMC-1 cells. CQ down-regulated caspase-1 (CASP1), MAPKs, and NF-κB levels enhanced by stimulation with PMA + A23187. Moreover, ear thickness in ear edema was suppressed following CQ treatment. CQ decreased CASP1 and NF-κB levels in the ear tissue. TSLP levels in the ear tissue and serum were reduced following CQ treatment. Collectively, the above findings elucidate that CQ inhibits the pro-inflammatory mechanisms of TSLP via the down-regulation of distinct intracellular signaling cascade in mast cells. Therefore, CQ may have protective roles against TSLP-mediated inflammatory disorders.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Thymus Gland
/
Signal Transduction
/
Chloroquine
/
Cytokines
/
Stromal Cells
/
Caspase 1
/
Caspase Inhibitors
/
Mast Cells
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Biomed Pharmacother
Year:
2021
Document Type:
Article
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