Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses.
J Vet Diagn Invest
; 33(3): 577-581, 2021 May.
Article
in English
| MEDLINE | ID: covidwho-1271943
ABSTRACT
The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Influenza A virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Real-Time Polymerase Chain Reaction
/
Influenza in Birds
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
J Vet Diagn Invest
Journal subject:
Veterinary Medicine
Year:
2021
Document Type:
Article
Affiliation country:
1040638721994810
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