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Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses.
Xiao, Yixin; Yang, Fan; Liu, Fumin; Yao, Hangping; Wu, Nanping; Wu, Haibo.
  • Xiao Y; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • Yang F; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • Liu F; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • Yao H; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • Wu N; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • Wu H; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
J Vet Diagn Invest ; 33(3): 577-581, 2021 May.
Article in English | MEDLINE | ID: covidwho-1271943
ABSTRACT
The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Influenza in Birds Type of study: Diagnostic study Limits: Animals Language: English Journal: J Vet Diagn Invest Journal subject: Veterinary Medicine Year: 2021 Document Type: Article Affiliation country: 1040638721994810

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Influenza in Birds Type of study: Diagnostic study Limits: Animals Language: English Journal: J Vet Diagn Invest Journal subject: Veterinary Medicine Year: 2021 Document Type: Article Affiliation country: 1040638721994810