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A host transcriptomic signature for identification of respiratory viral infections in the community.
Almansa, Raquel; Herrero-Rodríguez, Carmen; Martínez-Huélamo, Misericordia; Vicente-Andres, Maria Del Pilar; Nieto-Barbero, Jose Angel; Martín-Ballesteros, Miryam; Rodilla-Carvajal, Maria Del Mar; de la Fuente, Amanda; Ortega, Alicia; Alonso-Ramos, Maria Jesus; Wacker, James; Liesenfeld, Oliver; Sweeney, Timothy E; Bermejo-Martin, Jesús F; García-Ortiz, Luis.
  • Almansa R; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca (IBSAL), Gerencia Regional de Salud, Salamanca, Spain.
  • Herrero-Rodríguez C; Hospital Universitario Río Hortega, Gerencia Regional de Salud, Valladolid, Spain.
  • Martínez-Huélamo M; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain.
  • Vicente-Andres MDP; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Nieto-Barbero JA; Unidad de Investigación en Atención Primaria de Salamanca (APISAL), Instituto de investigación Biomédica de Salamanca (IBSAL), Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Martín-Ballesteros M; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Rodilla-Carvajal MDM; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • de la Fuente A; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Ortega A; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Alonso-Ramos MJ; Servicio de Urgencias de Atención Primaria de Salamanca (SUAP). Gerencia de Atención Primaria de Salamanca, Gerencia Regional de salud de Castilla y León (SACyL), Salamanca, Spain.
  • Wacker J; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca (IBSAL), Gerencia Regional de Salud, Salamanca, Spain.
  • Liesenfeld O; Hospital Universitario Río Hortega, Gerencia Regional de Salud, Valladolid, Spain.
  • Sweeney TE; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca (IBSAL), Gerencia Regional de Salud, Salamanca, Spain.
  • Bermejo-Martin JF; Hospital Universitario Río Hortega, Gerencia Regional de Salud, Valladolid, Spain.
  • García-Ortiz L; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca (IBSAL), Gerencia Regional de Salud, Salamanca, Spain.
Eur J Clin Invest ; 51(12): e13626, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1273086
Semantic information from SemMedBD (by NLM)
1. Respiratory viruses PROCESS_OF Community
Subject
Respiratory viruses
Predicate
PROCESS_OF
Object
Community
2. Blood LOCATION_OF HK3
Subject
Blood
Predicate
LOCATION_OF
Object
HK3
3. Blood LOCATION_OF Cathepsins B|CTSB
Subject
Blood
Predicate
LOCATION_OF
Object
Cathepsins B|CTSB
4. Blood LOCATION_OF JUP gene|JUP
Subject
Blood
Predicate
LOCATION_OF
Object
JUP gene|JUP
5. Blood LOCATION_OF GPAA1 gene|GPAA1
Subject
Blood
Predicate
LOCATION_OF
Object
GPAA1 gene|GPAA1
6. Blood LOCATION_OF IFI27 gene|IFI27
Subject
Blood
Predicate
LOCATION_OF
Object
IFI27 gene|IFI27
7. Blood LOCATION_OF TNIP1 gene|TNIP1
Subject
Blood
Predicate
LOCATION_OF
Object
TNIP1 gene|TNIP1
8. Blood LOCATION_OF LAX1 gene|LAX1
Subject
Blood
Predicate
LOCATION_OF
Object
LAX1 gene|LAX1
9. Sign or Symptom PROCESS_OF Patients
Subject
Sign or Symptom
Predicate
PROCESS_OF
Object
Patients
10. Virus Diseases COEXISTS_WITH Severe (severity modifier)
Subject
Virus Diseases
Predicate
COEXISTS_WITH
Object
Severe (severity modifier)
11. Viral respiratory infection PROCESS_OF Community
Subject
Viral respiratory infection
Predicate
PROCESS_OF
Object
Community
12. Respiratory viruses PROCESS_OF Community
Subject
Respiratory viruses
Predicate
PROCESS_OF
Object
Community
13. Blood LOCATION_OF HK3
Subject
Blood
Predicate
LOCATION_OF
Object
HK3
14. Blood LOCATION_OF Cathepsins B|CTSB
Subject
Blood
Predicate
LOCATION_OF
Object
Cathepsins B|CTSB
15. Blood LOCATION_OF JUP gene|JUP
Subject
Blood
Predicate
LOCATION_OF
Object
JUP gene|JUP
16. Blood LOCATION_OF GPAA1 gene|GPAA1
Subject
Blood
Predicate
LOCATION_OF
Object
GPAA1 gene|GPAA1
17. Blood LOCATION_OF IFI27 gene|IFI27
Subject
Blood
Predicate
LOCATION_OF
Object
IFI27 gene|IFI27
18. Blood LOCATION_OF TNIP1 gene|TNIP1
Subject
Blood
Predicate
LOCATION_OF
Object
TNIP1 gene|TNIP1
19. Blood LOCATION_OF LAX1 gene|LAX1
Subject
Blood
Predicate
LOCATION_OF
Object
LAX1 gene|LAX1
20. Sign or Symptom PROCESS_OF Patients
Subject
Sign or Symptom
Predicate
PROCESS_OF
Object
Patients
21. Virus Diseases COEXISTS_WITH Severe (severity modifier)
Subject
Virus Diseases
Predicate
COEXISTS_WITH
Object
Severe (severity modifier)
22. Viral respiratory infection PROCESS_OF Community
Subject
Viral respiratory infection
Predicate
PROCESS_OF
Object
Community
ABSTRACT

BACKGROUND:

Fever-7 is a test evaluating host mRNA expression levels of IFI27, JUP, LAX, HK3, TNIP1, GPAA1 and CTSB in blood able to detect viral infections. This test has been validated mostly in hospital settings. Here we have evaluated Fever-7 to identify the presence of respiratory viral infections in a Community Health Center.

METHODS:

A prospective study was conducted in the "Servicio de Urgencias de Atención Primaria" in Salamanca, Spain. Patients with clinical signs of respiratory infection and at least one point in the National Early Warning Score were recruited. Fever-7 mRNAs were profiled on a Nanostring nCounter® SPRINT instrument from blood collected upon patient enrolment. Viral diagnosis was performed on nasopharyngeal aspirates (NPAs) using the Biofire-RP2 panel.

RESULTS:

A respiratory virus was detected in the NPAs of 66 of the 100 patients enrolled. Median National Early Warning Score was 7 in the group with no virus detected and 6.5 in the group with a respiratory viral infection (P > .05). The Fever-7 score yielded an overall AUC of 0.81 to predict a positive viral syndromic test. The optimal operating point for the Fever-7 score yielded a sensitivity of 82% with a specificity of 71%. Multivariate analysis showed that Fever-7 was a robust marker of viral infection independently of age, sex, major comorbidities and disease severity at presentation (OR [CI95%], 3.73 [2.14-6.51], P < .001).

CONCLUSIONS:

Fever-7 is a promising host immune mRNA signature for the early identification of a respiratory viral infection in the community.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Language: English Journal: Eur J Clin Invest Clinical aspect: Diagnosis / Prediction / Prognosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Language: English Journal: Eur J Clin Invest Clinical aspect: Diagnosis / Prediction / Prognosis Year: 2021
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