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Studying SARS-CoV-2 with Fluorescence Microscopy.
Putlyaeva, Lidia V; Lukyanov, Konstantin A.
  • Putlyaeva LV; Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.
  • Lukyanov KA; Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.
Int J Mol Sci ; 22(12)2021 Jun 18.
Article in English | MEDLINE | ID: covidwho-1273462
ABSTRACT
The COVID-19 pandemic caused by SARS-CoV-2 coronavirus deeply affected the world community. It gave a strong impetus to the development of not only approaches to diagnostics and therapy, but also fundamental research of the molecular biology of this virus. Fluorescence microscopy is a powerful technology enabling detailed investigation of virus-cell interactions in fixed and live samples with high specificity. While spatial resolution of conventional fluorescence microscopy is not sufficient to resolve all virus-related structures, super-resolution fluorescence microscopy can solve this problem. In this paper, we review the use of fluorescence microscopy to study SARS-CoV-2 and related viruses. The prospects for the application of the recently developed advanced methods of fluorescence labeling and microscopy-which in our opinion can provide important information about the molecular biology of SARS-CoV-2-are discussed.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / Microscopy, Fluorescence Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Ijms22126558

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / Microscopy, Fluorescence Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Ijms22126558