Molecular and biological characterization of influenza A viruses isolated from human fecal samples.
Infect Genet Evol
; 93: 104972, 2021 09.
Article
in English
| MEDLINE | ID: covidwho-1274364
ABSTRACT
Human influenza viruses are occasionally detected in the stools of influenza patients. OBJECTIVES:
Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.METHODS:
Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.RESULTS:
Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.CONCLUSION:
The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Influenza A virus
/
Influenza, Human
Type of study:
Experimental Studies
/
Observational study
Limits:
Adolescent
/
Adult
/
Aged
/
Humans
/
Middle aged
/
Young adult
Country/Region as subject:
Asia
Language:
English
Journal:
Infect Genet Evol
Journal subject:
Biology
/
Communicable Diseases
/
Genetics
Year:
2021
Document Type:
Article
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