Comparison of two assays to detect IgG antibodies to the receptor binding domain of SARSCoV2 as a surrogate marker for assessing neutralizing antibodies in COVID-19 patients.
Int J Infect Dis
; 109: 85-89, 2021 Aug.
Article
in English
| MEDLINE | ID: covidwho-1275381
ABSTRACT
BACKGROUND:
Neutralizing antibodies (NAbs) are important for protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reinfection. In this study, two assays that are correlated with NAbs were compared the haemagglutination test (HAT) and the surrogate virus neutralization test (sVNT).METHODS:
The specificity of the HAT was compared with the sVNT, and the sensitivity and persistence of antibodies in patients with varying severity of illness was assessed in a cohort of 71 patients at 4-6 weeks and 13-16 weeks. The kinetics were assessed in the first, second, and third weeks in patients with varying severity of acute illness.RESULTS:
The specificity of the HAT was >99%, and sensitivity was similar to the sVNT. The levels of HAT were significantly and positively correlated with those of the sVNT (Spearman's r = 0.78, P < 0.0001). Patients with moderate and severe illness had higher HAT titres when compared to those with mild illness. Six of seven patients with severe illness had a titre of >1640 during the second week of illness, whereas only five of 31 patients with a mild illness had a titre of >1160 in the second week of illness.CONCLUSIONS:
Since the HAT is a simple and very cheap assay to perform, it would be ideal to use as an indicator of NAbs in resource-poor settings.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
SARS-CoV-2
/
COVID-19
Type of study:
Cohort study
/
Diagnostic study
/
Observational study
/
Prognostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
Int J Infect Dis
Journal subject:
Communicable Diseases
Year:
2021
Document Type:
Article
Affiliation country:
J.ijid.2021.06.031
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