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Comparison of two assays to detect IgG antibodies to the receptor binding domain of SARS­CoV­2 as a surrogate marker for assessing neutralizing antibodies in COVID-19 patients.
Kamaladasa, Achala; Gunasekara, Banuri; Jeewandara, Chandima; Jayathilaka, Deshni; Wijewickrama, Ananda; Guruge, Dinuka; Wijayamuni, Ruwan; Tan, T K; Ogg, Graham S; Townsend, Alain; Malavige, Gathsaurie Neelika.
  • Kamaladasa A; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
  • Gunasekara B; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
  • Jeewandara C; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
  • Jayathilaka D; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
  • Wijewickrama A; National Institute of Infectious Diseases, Angoda, Sri Lanka.
  • Guruge D; Colombo Municipality Council, Sri Lanka.
  • Wijayamuni R; Colombo Municipality Council, Sri Lanka.
  • Tan TK; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
  • Ogg GS; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
  • Townsend A; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
  • Malavige GN; AICBU, Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. Electronic address: gathsaurie.malavige@ndm.ox.a
Int J Infect Dis ; 109: 85-89, 2021 Aug.
Article in English | MEDLINE | ID: covidwho-1275381
ABSTRACT

BACKGROUND:

Neutralizing antibodies (NAbs) are important for protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reinfection. In this study, two assays that are correlated with NAbs were compared the haemagglutination test (HAT) and the surrogate virus neutralization test (sVNT).

METHODS:

The specificity of the HAT was compared with the sVNT, and the sensitivity and persistence of antibodies in patients with varying severity of illness was assessed in a cohort of 71 patients at 4-6 weeks and 13-16 weeks. The kinetics were assessed in the first, second, and third weeks in patients with varying severity of acute illness.

RESULTS:

The specificity of the HAT was >99%, and sensitivity was similar to the sVNT. The levels of HAT were significantly and positively correlated with those of the sVNT (Spearman's r = 0.78, P < 0.0001). Patients with moderate and severe illness had higher HAT titres when compared to those with mild illness. Six of seven patients with severe illness had a titre of >1640 during the second week of illness, whereas only five of 31 patients with a mild illness had a titre of >1160 in the second week of illness.

CONCLUSIONS:

Since the HAT is a simple and very cheap assay to perform, it would be ideal to use as an indicator of NAbs in resource-poor settings.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Topics: Variants Limits: Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: J.ijid.2021.06.031

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Topics: Variants Limits: Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: J.ijid.2021.06.031