Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium.
STAR Protoc
; 2(3): 100663, 2021 09 17.
Article
in English
| MEDLINE | ID: covidwho-1275773
ABSTRACT
Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differentiation of NHBE cells in air-liquid interface mimics an in vivo airway and provides an in vitro model for studying SARS-CoV-2 infection. We also describe a protocol for detecting proteins in the sectioned epithelium for detailing SARS-CoV-2 infection-induced pathobiology with a vertical view.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Bronchi
/
Pulmonary Disease, Chronic Obstructive
/
Epithelium
/
Coronavirus Nucleocapsid Proteins
/
SARS-CoV-2
/
COVID-19
Topics:
Long Covid
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2021
Document Type:
Article
Affiliation country:
J.xpro.2021.100663
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