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Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium.
Osan, Jaspreet Kaur; DeMontigny, Beth Ann; Mehedi, Masfique.
  • Osan JK; Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58202, USA.
  • DeMontigny BA; Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58202, USA.
  • Mehedi M; Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58202, USA.
STAR Protoc ; 2(3): 100663, 2021 09 17.
Article in English | MEDLINE | ID: covidwho-1275773
ABSTRACT
Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differentiation of NHBE cells in air-liquid interface mimics an in vivo airway and provides an in vitro model for studying SARS-CoV-2 infection. We also describe a protocol for detecting proteins in the sectioned epithelium for detailing SARS-CoV-2 infection-induced pathobiology with a vertical view.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bronchi / Pulmonary Disease, Chronic Obstructive / Epithelium / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 Topics: Long Covid Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100663

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bronchi / Pulmonary Disease, Chronic Obstructive / Epithelium / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 Topics: Long Covid Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100663