A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients.
STAR Protoc
; 2(3): 100671, 2021 09 17.
Article
in English
| MEDLINE | ID: covidwho-1275774
ABSTRACT
One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Neutralizing
/
Spike Glycoprotein, Coronavirus
/
Flow Cytometry
/
COVID-19 Serological Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2021
Document Type:
Article
Affiliation country:
J.xpro.2021.100671
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