Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing.
CRISPR J
; 4(3): 392-399, 2021 06.
Article
in English
| MEDLINE | ID: covidwho-1276110
ABSTRACT
Rapid and clinically sensitive detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) play an important role in the contact tracing and containment of the COVID-19 pandemic. A recently developed field-deployable clustered regularly interspaced short palindromic repeats (CRISPR) detection assay with lateral flow strips shows promise for point-of-care detection of SARS-CoV-2. However, the limit of detection of paper strip-based assays (10-100 copies/µL) is much lower than that of fluorescence-based detection methods. In this study, we developed an easy-readout and sensitive enhanced (ERASE) strip to visualize the results of CRISPR detection and improve the sensitivity to 1 copy/µL with an unambiguous easy-read result. Using 649 clinical samples from blind specimens collected from patients in China, we validated our ERASE assay for SARS-CoV-2 RNA detection with 90.67% positive predictive agreement and 99.21% negative predictive agreement. In conclusion, our study provided a customized CRISPR strip for use in a simple, rapid, ultrasensitive, and highly specific assay for SARS-CoV-2 detection. (Clinical Trial Registration number 2020-008-01; [2020]IEC(ZD01); PJ-NBEY-2020-009-01; 2020#34).
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
CRISPR-Cas Systems
/
Point-of-Care Testing
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Prognostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
CRISPR J
Year:
2021
Document Type:
Article
Affiliation country:
Crispr.2020.0138
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