Your browser doesn't support javascript.
Clinical Evaluation of the Abbott Alinity SARS-CoV-2 Spike-Specific Quantitative IgG and IgM Assays among Infected, Recovered, and Vaccinated Groups.
Narasimhan, Madhusudhanan; Mahimainathan, Lenin; Araj, Ellen; Clark, Andrew E; Markantonis, John; Green, Allen; Xu, Jing; SoRelle, Jeffrey A; Alexis, Charles; Fankhauser, Kimberly; Parikh, Hiren; Wilkinson, Kathleen; Reczek, Annika; Kopplin, Noa; Yekkaluri, Sruthi; Balani, Jyoti; Thomas, Abey; Singal, Amit G; Sarode, Ravi; Muthukumar, Alagarraju.
  • Narasimhan M; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Mahimainathan L; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Araj E; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Clark AE; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Markantonis J; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Green A; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Xu J; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • SoRelle JA; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Alexis C; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Fankhauser K; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Parikh H; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Wilkinson K; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Reczek A; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Kopplin N; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Yekkaluri S; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Balani J; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Thomas A; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Singal AG; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Sarode R; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
  • Muthukumar A; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
J Clin Microbiol ; 59(7): e0038821, 2021 06 18.
Article in English | MEDLINE | ID: covidwho-1276887
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
The coronavirus disease 19 (COVID-19) pandemic continues to impose a significant burden on global health infrastructure. While identification and containment of new cases remain important, laboratories must now pivot and consider an assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity in the setting of the recent availability of multiple COVID-19 vaccines. Here, we have utilized the latest Abbott Alinity semiquantitative IgM and quantitative IgG spike protein (SP) serology assays (IgMSP and IgGSP) in combination with Abbott Alinity IgG nucleocapsid (NC) antibody test (IgGNC) to assess antibody responses in a cohort of 1,236 unique participants comprised of naive, SARS-CoV-2-infected, and vaccinated (including both naive and recovered) individuals. The IgMSP and IgGSP assays were highly specific (100%) with no cross-reactivity to archived samples collected prior to the emergence of SARS-CoV-2, including those from individuals with seasonal coronavirus infections. Clinical sensitivity was 96% after 15 days for both IgMSP and IgGSP assays individually. When considered together, the sensitivity was 100%. A combination of NC- and SP-specific serologic assays clearly differentiated naive, SARS-CoV-2-infected, and vaccine-related immune responses. Vaccination resulted in a significant increase in IgGSP and IgMSP values, with a major rise in IgGSP following the booster (second) dose in the naive group. In contrast, SARS-CoV-2-recovered individuals had several-fold higher IgGSP responses than naive following the primary dose, with a comparatively dampened response following the booster. This work illustrates the strong clinical performance of these new serological assays and their utility in evaluating and distinguishing serological responses to infection and vaccination.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: J Clin Microbiol Year: 2021 Document Type: Article Affiliation country: JCM.00388-21

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: J Clin Microbiol Year: 2021 Document Type: Article Affiliation country: JCM.00388-21