Laboratory Worker Self-Contamination with Noninfectious SARS-CoV-2 DNA Can Result in False-Positive Reverse Transcriptase PCR-Based Surveillance Testing.

Montgomery, Theresa L; Paavola, Michelle; Bruce, Emily A; Botten, Jason W; Crothers, Jessica W; Krementsov, Dimitry N

Montgomery, Theresa L; Paavola, Michelle; Bruce, Emily A; Botten, Jason W; Crothers, Jessica W; Krementsov, Dimitry N.

Montgomery TL; Department of Biomedical and Health Sciences, College of Nursing and Health Sciences, University of Vermont, Burlington, Vermont, USA.
Paavola M; Center for Health and Wellbeing, University of Vermont, Burlington, Vermont, USA.
Bruce EA; Department of Microbiology & Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Botten JW; Translational Global Infectious Disease Research Center, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Crothers JW; Department of Microbiology & Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Krementsov DN; Translational Global Infectious Disease Research Center, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.

J Clin Microbiol ; 59(7): e0072321, 2021 06 18.

Article in English | MEDLINE | ID: covidwho-1276890

Subject(s)

COVID-19; SARS-CoV-2; Clinical Laboratory Techniques; DNA; Humans; Laboratories; Reverse Transcriptase Polymerase Chain Reaction

Keywords

RT-PCR; SARS-CoV-2; cDNA; contamination; coronavirus; false positive

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Clin Microbiol Year: 2021 Document Type: Article Affiliation country: JCM.00723-21

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