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Rapid, simplified whole blood-based multiparameter assay to quantify and phenotype SARS-CoV-2-specific T-cells.
Riou, Catherine; Schäfer, Georgia; du Bruyn, Elsa; Goliath, Rene T; Stek, Cari; Mou, Huihui; Hung, Deli; Wilkinson, Katalin A; Wilkinson, Robert J.
  • Riou C; Wellcome Centre for Infectious Disease Research in Africa and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa cr.riou@uct.ac.za.
  • Schäfer G; Division of Medical Virology, Dept of Pathology, University of Cape Town, Observatory, South Africa.
  • du Bruyn E; Wellcome Centre for Infectious Disease Research in Africa and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.
  • Goliath RT; International Centre for Genetic Engineering and Biotechnology (ICGEB) Cape Town, Observatory, South Africa.
  • Stek C; Division of Medical Biochemistry and Structural Biology, Dept of Integrative Biomedical Sciences, University of Cape Town, Observatory, South Africa.
  • Mou H; Wellcome Centre for Infectious Disease Research in Africa and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.
  • Hung D; Dept of Medicine, University of Cape Town, Observatory, South Africa.
  • Wilkinson KA; Wellcome Centre for Infectious Disease Research in Africa and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.
  • Wilkinson RJ; Wellcome Centre for Infectious Disease Research in Africa and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.
Eur Respir J ; 59(1)2022 01.
Article in English | MEDLINE | ID: covidwho-1277909
Preprint
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ABSTRACT

BACKGROUND:

Rapid tests to evaluate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T-cell responses are urgently needed to decipher protective immunity and aid monitoring vaccine-induced immunity.

METHODS:

Using a rapid whole blood assay requiring a minimal amount of blood, we measured qualitatively and quantitatively SARS-CoV-2-specific CD4 T-cell responses in 31 healthcare workers using flow cytometry.

RESULTS:

100% of COVID-19 convalescent participants displayed a detectable SARS-CoV-2-specific CD4 T-cell response. SARS-CoV-2-responding cells were also detected in 40.9% of participants with no COVID-19-associated symptoms or who tested PCR-negative. Phenotypic assessment indicated that, in COVID-19 convalescent participants, SARS-CoV-2 CD4 responses displayed an early differentiated memory phenotype with limited capacity to produce interferon (IFN)-γ. Conversely, in participants with no reported symptoms, SARS-CoV-2 CD4 responses were enriched in late differentiated cells, coexpressing IFN-γ and tumour necrosis factor-α and also Granzyme B.

CONCLUSIONS:

This proof-of-concept study presents a scalable alternative to peripheral blood mononuclear cell-based assays to enumerate and phenotype SARS-CoV-2-responding T-cells, thus representing a practical tool to monitor adaptive immunity due to natural infection or vaccine trials.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Experimental Studies / Qualitative research Topics: Long Covid / Vaccines Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: 13993003.00285-2021

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Experimental Studies / Qualitative research Topics: Long Covid / Vaccines Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: 13993003.00285-2021