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CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2.
Cao, Yiren; Wu, Jinjun; Pang, Bo; Zhang, Hongquan; Le, X Chris.
  • Cao Y; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
  • Wu J; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
  • Pang B; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
  • Zhang H; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
  • Le XC; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. hongquan@ualberta.ca xc.le@ualberta.ca.
Chem Commun (Camb) ; 57(56): 6871-6874, 2021 Jul 13.
Article in English | MEDLINE | ID: covidwho-1281748
ABSTRACT
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Metal Nanoparticles / CRISPR-Cas Systems / Genes, Viral / SARS-CoV-2 / COVID-19 / Gold Type of study: Diagnostic study / Randomized controlled trials Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Metal Nanoparticles / CRISPR-Cas Systems / Genes, Viral / SARS-CoV-2 / COVID-19 / Gold Type of study: Diagnostic study / Randomized controlled trials Language: English Journal: Chem Commun (Camb) Journal subject: Chemistry Year: 2021 Document Type: Article